1. Adipocytes sequester daunorubicin (DNR).
Adipocytes sequester daunorubicin (DNR). A, Confocal microscopy pictures of adipocytes treated with different concentrations of daunorubicin (red) for 4 hours. Scale bar, 50 μm. B, Viable BV173 ALL cells cultured for 72 hours in media in which 100 nmol/L daunorubicin had been preincubated for the indicated time over no feeder (open), fibroblasts (hatched), or adipocytes (solid bars; dotted line indicates initial cell number; n = 4). C, Viable BV173 ALL cells cultured for 72 hours in media that had been preincubated over no feeder (open) or adipocytes (solid bars) for 48 hours with various initial daunorubicin concentrations. P values indicate comparisons to no feeder conditions (n = 3). D, Viable ALL cells after 72 hours in culture with lysates of adipocytes that had been preincubated with DNR for 48 hours. P values indicate comparisons to the 0 daunorubicin lysate condition (n = 3 for 100 nmol/L, n = 6 for all other doses). E and F, Media doxorubicin (E) and mitoxantrone (F) concentrations after 16 hour incubation of each drug at the indicated concentrations alone (open) or over 3T3-L1 adipocytes (closed bars; n = 4–6). *, P < 0.05; **, P < 0.01; ***, P <
Xia Sheng et al. Mol Cancer Res 2017;15:
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