1. CypD-deficient mice are susceptible to Mtb infection.
CypD-deficient mice are susceptible to Mtb infection. (A) Bacterial burden in the lung, spleen, and liver of WT and Ppif−/− mice (n = 4 to 5 per group) at 7, 14, and 21 days after intravenous infection with 106 H37Rv (two-way ANOVA). (B) Survival of WT and Ppif−/− mice (n = 10 per group) after intravenous infection with 106 H37Rv (log-rank test). (C) Bacterial burden in the lungs after 14, 35, and 90 days of aerosol infection with ~50 H37Rv (n = 5 per group) (two-way ANOVA). (D) Survival of WT and Ppif−/− mice after aersolized infection with ~50 H37Rv (n = 10 per group). *P < 0.05 (log-rank test). (E and F) Representative gross pathology (E) and histology (H&E staining) and inflammatory scores (F) of the lungs of WT and Ppif−/− mice at day 90 after aerosol infection with ~50 H37Rv. Lymphocytes (black arrows) and macrophages (dashed arrows). *P < 0.05 (unpaired t test) (G) Total number of CD4+ (top) and CD8+ T cells (bottom) in the lungs of WT and Ppif−/− mice (n = 5 per group) at day 90 after aerosol infection with ~50 H37Rv. *P < 0.05 (unpaired t test). Data are representative of two independent experiments.
Fanny Tzelepis et al. Sci. Immunol. 2018;3:eaar4135
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