1. Telomere cohesion is established in S phase in cohesin-ring-depleted cells.
Telomere cohesion is established in S phase in cohesin-ring-depleted cells. HeLa.I.2.11 cells were synchronized by a double thymidine block, treated with siRNA against GFP, SA1, SA2, Scc1 or Smc3 and analyzed by (A) FACS, (B) telomere FISH with a telomere 16ptelo (green), or (C) centromere FISH with a 6cen (red) probe 4 hours after release from the second thymidine block. DNA was stained with DAPI (blue). Scale bar: 5 µm. (D,E) Graphical representation of the frequency of telomere (from B; n = 514 cells or more each) and centromere (from C; n = 441 cells or more each) doublets in S phase, respectively. Values are means ± s.e.m., derived from two independent experiments. (F) Graphical representation of the fold increase in telomere (from D) and centromere (from E) doublets relative to the GFP siRNA control.
Kamlesh K. Bisht et al. J Cell Sci 2013;126:
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