1. Western blot of MDA-modified proteins in FVB control, OVE26 diabetic, and catalase-protected OVE26C diabetic hearts.
Western blot of MDA-modified proteins in FVB control, OVE26 diabetic, and catalase-protected OVE26C diabetic hearts. The intensity of the 50-kDa protein on this blot was elevated 2.5-fold in OVE26 samples (P < 0.02) compared with FVB samples. The intensity of FVB and OVE26C immunostaining was not significantly different. MDA-modified proteins were recognized with polyclonal goat anti-MDA antibody and visualized with peroxidase-conjugated anti-goat antibody. Statistical analysis was performed by ANOVA and Tukey’s post hoc test with three animals per group. Band intensities were normalized to β-actin intensity.
Gang Ye et al. Diabetes 2004;53:
©2004 by American Diabetes Association