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Identification of crab proteins that elicit IgE reactivity in snow crab–processing workers  Beth V. Gill, MD, Timothy R. Rice, André Cartier, MD, Denise.

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Presentation on theme: "Identification of crab proteins that elicit IgE reactivity in snow crab–processing workers  Beth V. Gill, MD, Timothy R. Rice, André Cartier, MD, Denise."— Presentation transcript:

1 Identification of crab proteins that elicit IgE reactivity in snow crab–processing workers 
Beth V. Gill, MD, Timothy R. Rice, André Cartier, MD, Denise Gautrin, MD, Barbara Neis, PhD, Lise Horth-Susin, RRT, Michael Jong, MD, Mark Swanson, PhD, Samuel B. Lehrer, PhD  Journal of Allergy and Clinical Immunology  Volume 124, Issue 5, Pages (November 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 RAST values (percentage IgE binding) of snow crab workers to CM (red) and CW (blue) extracts. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Autoradiograph at day 9 of all sera incubated against CM (upper panel) and CW (lower panel) extracts. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Examples of IgE antibody reactivity of individual sera that reacted to similar (A) or different (B) proteins in CM (left column) and CW (right column) extracts. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Allergologram of sera against CM (A) and CW (B) extracts. The shown shadings correspond to high, medium, and low individual sera binding (labeled by row) to individual proteins (labeled by column). Serum sample 25 is from a nonexposed control subject with negative RAST results. The results are tabulated in Table I. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Protein bands (A) and reactivity of pooled IgE-reactive snow crab workers' sera (B) against crab meat, water and filter extracts. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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