1. Flow chamber Poiseuille flowD
t: shear stress. 10 dynes/cm2 (1N/m2 or 1Pa)
D: flow channel height.
b: flow channel width.
Q: flow rate. m: viscosity N.s/m2
From Jacobs et al 1998 J. Biomechanics

2. Chamber dimensions Big flow chamber: Slide: chamber:
(Jacobs et al., 1998, You et al., 2000)
(Li et al., 2004, NIH Grant)
(IU, Whitehaus Precision Machining, , Don Whitehaus )
Small flow chamber:
Slide:
chamber:

3. Sample Calculation
NOTE: This is for 1Pa or 10 dynes/cm2. Using a Hamilton Syringe Gastight # 1750 (500 ml, 60mm scale length) this is roughly 17mm for a full stroke.