1. Variable number of CD4-CTL precursors across donors.
Variable number of CD4-CTL precursors across donors. (A) tSNE 2D plot of single-cell RNA-seq data obtained using the Seurat software package for donors #2 (left) and #1 (right) from the 3′ transcriptome of single cells (RNA-seq carried out using the 10× genomics platform) for the most variable transcripts (1799, donor #2; 1569, donor #1). (B) tSNE 2D plots (left) and violin plots (right) of single-cell RNA-seq data show the expression [log2(UPM + 1) expression; UPM, UMI per million] of the indicated differentially expressed transcripts (SCDE and MAST analysis, Benjamini-Hochberg adjusted P < 0.05 and ≥2-fold change) for the indicated donors. Precursor cluster is marked with a black dashed line. The shapes in violin plots represent the distribution of cells based on their log2(UPM + 1) expression values (y axis). The color scale represents the expression of log2(UPM + 1) (tSNE 2D plots) and mean expression (violin plots). (C) Violin plots show the single-cell expression pattern of the indicated differentially expressed transcripts between IL-7Rhigh TEMRA (CD4-CTL precursors) and IL-7R− TEMRA (CD4-CTL effectors) subsets. The shapes represent the distribution of cells based on their log2(TPM + 1) expression values (y axis). Color scale shows the mean expression values.
Veena S. Patil et al. Sci. Immunol. 2018;3:eaan8664
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