Presentation is loading. Please wait.

Presentation is loading. Please wait.

Figure 4. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: growth, viability and ... Figure 4. MTase JHP1050 inactivation.

Published byJan Jarosz Modified over 5 years ago

Similar presentations

Presentation on theme: "Figure 4. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: growth, viability and ... Figure 4. MTase JHP1050 inactivation."— Presentation transcript:

1 Figure 4. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: growth, viability and ... Figure 4. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: growth, viability and morphology. (A) Growth curves for four wild type strains and mutants and for the complemented strains J99-compl, compl and BCM-300-compl were measured for 72 h. The doubling time for H. pylori was calculated to be 3.87 h (46). Statistics: two-way ANOVA, *P < 0.05, **P < 0.01, ****P < , bars: SD. (B) Viability of the strains was studied using epifluorescence microscopy after live/dead staining. Statistics: two-way ANOVA, *P < 0.05, bars: SD. (C) Bacterial morphology was quantitated from epifluorescence microscopy pictures using ImageJ. A value of 0 represents completely elongated bacteria, while a value of 1 means a complete circle (coccoid bacteria). Statistics: one-way ANOVA, **P < 0.01, bars: 95% confidence interval (CI). Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, Volume 47, Issue 5, 09 January 2019, Pages 2336–2348, The content of this slide may be subject to copyright: please see the slide notes for details.

2 Figure 3. Quantification of transcript amounts of jhp0832 in H
Figure 3. Quantification of transcript amounts of jhp0832 in H. pylori strains J99, J99-mut and the J99 mutants with ... Figure 3. Quantification of transcript amounts of jhp0832 in H. pylori strains J99, J99-mut and the J99 mutants with point mutations within the GCGC motifs. qPCR results are represented in the right panel, three different biological replicates were performed. Statistics: One-way ANOVA, **P < 0.01, ****P < , bars: SD. Legend: The jhp0832 gene is shown as a gray arrow. The predicted promoter is represented by a black arrow. Crosses represent methylated motifs while vertical lines mean unmethylated motifs (due to site-directed mutation, or to inactivation of the MTase in strain J99-mut). The GCGC motifs appear in blue and the motifs mutated to GAGC are colored in pink. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, Volume 47, Issue 5, 09 January 2019, Pages 2336–2348, The content of this slide may be subject to copyright: please see the slide notes for details.

3 Figure 2. Graphical representation of the percentage of genes with GCGC motifs 500 bp of sequence upstream of the start ... Figure 2. Graphical representation of the percentage of genes with GCGC motifs 500 bp of sequence upstream of the start codon for differentially expressed genes (DEGs) and genes not showing differential expression (Non-DEGs). Non-DEGs versus DEGs in BCM-300 (A) and J99 (C). DEGs in J99 shared with BCM-300 versus the rest of the J99 genes (B). DEGs in J99 shared with BCM-300 versus the rest of the J99 DEGs (all DEGs) (D). Statistics: Chi-square, *P < 0.05, **P < 0.01. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, Volume 47, Issue 5, 09 January 2019, Pages 2336–2348, The content of this slide may be subject to copyright: please see the slide notes for details.

4 Figure 1. Phylogenetic analysis of the GCGC-specific R–M system JHP1050/1049 (M.Hpy99III/Hpy99III) in H. pylori. ... Figure 1. Phylogenetic analysis of the GCGC-specific R–M system JHP1050/1049 (M.Hpy99III/Hpy99III) in H. pylori. Neighbour-Joining trees based on the nucleotide sequences of MTase M.Hpy99III (A) and REase Hpy99III (B). In both cases, strain symbols are colored according to the phylogeographic population assignment based on seven gene MLST and STRUCTURE analysis (see right panel for color coding). Filled circles represent strains without REase gene, while unfilled circles are used for strains containing both MTase and REase genes. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, Volume 47, Issue 5, 09 January 2019, Pages 2336–2348, The content of this slide may be subject to copyright: please see the slide notes for details.

5 Figure 5. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: natural competence, ... Figure 5. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: natural competence, resistance to copper, and adherence to host cells. (A) Transformation experiments were performed with 1 μg/ml of gDNA. Statistics: Welch’s unpaired t test, **P < 0.01, ****P < , bars: SD. (B) The growth of J99 wild type, J99-mut, BCM-300 wild type and BCM-300-mut strains was measured 24 h post-inoculation after addition of different concentrations of copper sulfate to the cultures. Data was normalized to a control culture without copper. Statistics: One-Way ANOVA, **P < 0.01, ***P < 0.001, ****P < , bars: SD. (C) Adherence of H. pylori wild type and mutant strains to fixed AGS cells. Statistics: unpaired t-test, *P < 0.05, bars: SD. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, Volume 47, Issue 5, 09 January 2019, Pages 2336–2348, The content of this slide may be subject to copyright: please see the slide notes for details.

Download ppt "Figure 4. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: growth, viability and ... Figure 4. MTase JHP1050 inactivation."

Similar presentations

Figure 1. Unified models predicting gene regulation based on landscapes of gene-regulating factors. For each gene, position specific combinatorial patterns.

Figure 1. Unified models predicting gene regulation based on landscapes of gene-regulating factors. For each gene, position specific combinatorial patterns.
Figure 1. The flow chart illustrates the construction process of anti-CRISPRdb, and the information that users can obtain from anti-CRISPRdb. From: Anti-CRISPRdb:

Figure 1. The flow chart illustrates the construction process of anti-CRISPRdb, and the information that users can obtain from anti-CRISPRdb. From: Anti-CRISPRdb:
Figure 1: Necrobiosis lipoidica: Yellow-brown skin lesions with indurated borders located on both lower legs. Figure 1: Necrobiosis lipoidica: Yellow-brown.

Figure 1: Necrobiosis lipoidica: Yellow-brown skin lesions with indurated borders located on both lower legs. Figure 1: Necrobiosis lipoidica: Yellow-brown.
Figure 1. (A) The VEGF promoter PQS and scheme of G oxidation to OG, as well as (B) the proposed APE1-dependent pathway ... Figure 1. (A) The VEGF promoter.

Figure 1. (A) The VEGF promoter PQS and scheme of G oxidation to OG, as well as (B) the proposed APE1-dependent pathway ... Figure 1. (A) The VEGF promoter.
Figure 1. Circular taxonomy tree based on the species that were sequenced in our study. Unless provided in the caption above, the following copyright applies.

Figure 1. Circular taxonomy tree based on the species that were sequenced in our study. Unless provided in the caption above, the following copyright applies.
Figure 1. Overview of the workflow of NetworkAnalyst 3.0.

Figure 1. Overview of the workflow of NetworkAnalyst 3.0.
Figure 2. Status of rRNA methylations upon SAM depletion

Figure 2. Status of rRNA methylations upon SAM depletion
Figure 1: Axial T2 W images of penis showed a well-defined septated area of 2 cm in the posterior aspect of ... Figure 1: Axial T2 W images.

Figure 1: Axial T2 W images of penis showed a well-defined septated area of 2 cm in the posterior aspect of ... Figure 1: Axial T2 W images.
Figure 1. Effect of random T/A→dU/A substitutions on transcription by T7 RNAP using a 321 bp DNA transcription template ... Figure 1. Effect of random.

Figure 1. Effect of random T/A→dU/A substitutions on transcription by T7 RNAP using a 321 bp DNA transcription template ... Figure 1. Effect of random.
FIGURE 1 Histological diagnoses divided into 8-year time frames (n = 1208). Unless provided in the caption above, the following copyright applies to the.

FIGURE 1 Histological diagnoses divided into 8-year time frames (n = 1208). Unless provided in the caption above, the following copyright applies to the.
Figure 1. Position and number of NLS improves genome editing by AsCas12a, LbCas12a and FnoCas12a. (A) General schematic ... Figure 1. Position and number.

Figure 1. Position and number of NLS improves genome editing by AsCas12a, LbCas12a and FnoCas12a. (A) General schematic ... Figure 1. Position and number.
FIGURE 1 The effect of daprodustat on hemoglobin (Hgb) levels

FIGURE 1 The effect of daprodustat on hemoglobin (Hgb) levels
Figure 1. BRCA1-associated R-Loop accumulation at a non-coding region upstream of ESR1 locus. (A) Alignment of DRIP-seq ... Figure 1. BRCA1-associated.

Figure 1. BRCA1-associated R-Loop accumulation at a non-coding region upstream of ESR1 locus. (A) Alignment of DRIP-seq ... Figure 1. BRCA1-associated.
FIGURE 2 Responses to the question: regarding vasoactive drugs, does your centre use the following frequently, rarely ... FIGURE 2 Responses to the question:

FIGURE 2 Responses to the question: regarding vasoactive drugs, does your centre use the following frequently, rarely ... FIGURE 2 Responses to the question:
FIGURE 1 Maternal (A) urinary aldosterone; (B) plasma active renin; (C) urinary AGT concentrations; and (D) plasma AGT ... FIGURE 1 Maternal (A) urinary.

FIGURE 1 Maternal (A) urinary aldosterone; (B) plasma active renin; (C) urinary AGT concentrations; and (D) plasma AGT ... FIGURE 1 Maternal (A) urinary.
FIGURE 1 Flow chart of patient enrollment and exclusion

FIGURE 1 Flow chart of patient enrollment and exclusion
Figure 1. Ratios of observed to expected numbers of exon boundaries aligning to boundaries of domain and disorder ... Figure 1. Ratios of observed to expected.

Figure 1. Ratios of observed to expected numbers of exon boundaries aligning to boundaries of domain and disorder ... Figure 1. Ratios of observed to expected.
Figure 1. autoMLST workflow depicting placement and de novo mode

Figure 1. autoMLST workflow depicting placement and de novo mode
Fig. 1 Kaplan-Meier plot of cumulative incidence of cancer onset following dermatomyositis diagnosis stratified ... Anti-TIF1-Ab: anti-transcriptional.

Fig. 1 Kaplan-Meier plot of cumulative incidence of cancer onset following dermatomyositis diagnosis stratified ... Anti-TIF1-Ab: anti-transcriptional.
Figure 1 Flortaucipir PET MUBADA/PERSI SUVr at baseline, 9 and 18 months for individual subjects. Each subject is ... Figure 1 Flortaucipir PET MUBADA/PERSI.

Figure 1 Flortaucipir PET MUBADA/PERSI SUVr at baseline, 9 and 18 months for individual subjects. Each subject is ... Figure 1 Flortaucipir PET MUBADA/PERSI.

Similar presentations

Ads by Google