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Isolation and biochemical characterization of a thaumatin-like kiwi allergen  Marija Gavrović-Jankulović, PhDa, Tanja Ćirković, MSa, Olga Vučković, MDb,

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Presentation on theme: "Isolation and biochemical characterization of a thaumatin-like kiwi allergen  Marija Gavrović-Jankulović, PhDa, Tanja Ćirković, MSa, Olga Vučković, MDb,"— Presentation transcript:

1 Isolation and biochemical characterization of a thaumatin-like kiwi allergen 
Marija Gavrović-Jankulović, PhDa, Tanja Ćirković, MSa, Olga Vučković, MDb, Marina Atanasković-Marković, PhDc, Arnd Petersen, PhDd, Gordana Gojgić, PhDa, Lidija Burazer, MSb, Ratko M. Jankov, PhDa  Journal of Allergy and Clinical Immunology  Volume 110, Issue 5, Pages (November 2002) DOI: /mai Copyright © 2002 Mosby, Inc. Terms and Conditions

2 Fig. 1 Binding patterns of patients' IgE to self-prepared kiwi fruit extract after Western blotting. Lane m, Molecular weight marker; lane a, protein stained by means of CBB stain; lanes 1-7, individual patient sera; lane b, buffer control; lane n, normal human sera. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2002 Mosby, Inc. Terms and Conditions

3 Fig. 2 SDS-PAGE of isolated TLP. Lane m, Molecular weight markers; lane a, separation in reducing conditions; lane b, separation in nonreducing conditions and 2-dimensional PAGE immunoblot of isolated TLP; lane c, nitrocellulose stained with Ponceau S; lane d, IgE reactivity of pool of patients' sera. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2002 Mosby, Inc. Terms and Conditions

4 Fig. 3 Antifungal activity assay of TLP to brewer's yeast cells. Lane m, Molecular weight markers; lane a, TLP (50 μg/mL); lane b, cells incubated with TLP (50 μg/mL); lane c, control; lane d, BSA (50 μg/mL); lane e, cells incubated with BSA (50 μg/mL). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2002 Mosby, Inc. Terms and Conditions

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