1. Western blot analysis of histone H1.X.
Western blot analysis of histone H1.X. (A,B) shows Coomassie-bluestained SDS page gels obtained from a total lysate of C. elegans, the recombinant C. elegans proteins lamin, intermediate filament proteins A1 and B1 and recombinant H1.X. (C) shows a corresponding blot stained with the αH1.X-101 antibody and (D) shows a corresponding blot stained with the αH1.X-11 antibody. No antibody is reactive with intermediate filament proteins. Both antibodies recognize H1.X as a band with an apparent molecular mass of 31 kDa. αH1.X-11 is crossreactive with C. elegans lamin and a further not identified protein.
Monika A. Jedrusik et al. J Cell Sci 2002;115:
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