1. IFN-γ–producing cells in the spleen and lungs postimmunization.
IFN-γ–producing cells in the spleen and lungs postimmunization. After priming with QIV, C57BL/6 mice were immunized with different formulations, as described in Fig. 7, and the spleens and lungs of the animals were collected on day 66 to quantify the Ag-specific T cells in these organs. The cells isolated from the spleen (A–C) or the lungs (D–F) were stimulated with either the NP-derived peptide ASNENMETM (NP specific) (A and D), whole virus H1N1pdm09 (B and E), or the irrelevant peptide RAHYNIVTF (derived from human papillomavirus E7) (C and F), and the number of IFN-γ–producing cells was measured by ELISPOT. **p < 0.01, ***p < 0.001, ****p <
Angela Choi et al. ImmunoHorizons 2019;3:
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