1. PfSec13 does not co-localize with P. falciparum heterochromatin.
PfSec13 does not co-localize with P. falciparum heterochromatin. (A) IFAs of the PfSec13-GFP expressing parasites that were co-immunolabeled with α-GFP (green) and α-H3K9me3 (red) at different stages of the IDC. In 85% of the counted nuclei of all stages (n = 100), the H3K9me3 signal and PfSec13-GFP did not co-localize (right-hand panel). Scale bar: 2 µm. (B) In vivo imaging of parasites expressing both PfSec13-GFP and PfHP1-HA-mCherry (Flueck et al., 2009) during the IDC. Although PfSec13 (green) and PfHP1 (red) localized to the nuclear periphery they do not co-localize. Scale bar: 1 µm. (C) In vivo confocal microscopy of parasites expressing both PfSec13-GFP and PfHP1-HA-mCherry. Scale bar: 2 µm.
Noa Dahan-Pasternak et al. J Cell Sci 2013;126:
© Published by The Company of Biologists Ltd