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Analysis of the Warburg signature.

Published byΠαραμονος Ζάχος Modified over 5 years ago

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Presentation on theme: "Analysis of the Warburg signature."— Presentation transcript:

1 Analysis of the Warburg signature.
Analysis of the Warburg signature. (A) Quantification by qRT–PCR of transcripts associated with the glycolytic/hypoxic signatures and tested on RNA from total H1 tumor mass (total, n = 3) or from dissociated (diss, n = 4) tumor cells after exclusion of the infiltrating CD45+ population by magnetic bead cell sorting (ANOVA statistics P < ). (B) Dosage of lactate production in H1 R and VR tumor samples (n = 6–8; t test) from untreated or cysteamine (CEA)-treated mice when indicated. (C) Seahorse analysis of the in vitro–grown H1 R cell lines quantifying the effect of 500 μM cysteamine (CEA) extemporaneously added to the cell culture before ECAR measurement (ANOVA statistics P < ). (D) Effect of the addition of increasing concentrations of cysteamine (CEA) and pantothenate (PAN) to H1 cell cultures in vitro evaluated by crystal violet staining of cells (treated day 1 − control day 0)/(control day 1 − control day 0). (E) Growth of H1 tumors in mice treated or not with CEA ip (120 mg/kg) (n = 6, multiple t test). Caroline Giessner et al. LSA 2018;1:e © 2018 Naquet et al.

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