1. Validation of FGFR1 and PLXDC2 as BACE2 substrates in COS-1 overexpression system.
Validation of FGFR1 and PLXDC2 as BACE2 substrates in COS-1 overexpression system. (A) FGFR1 in lysates and medium of COS-1 cells overexpressing FGFR1 alone (lane 2), FGFR1 with BACE1 (lanes 3 and 4), and FGFR1 with BACE2 (lanes 5 and 6). Lanes 4 and 6 were treated with inhibitor CpJ (10 μM). Lane 1 was mock-transfected with an empty vector. Representative for three experiments. (B) PLXDC2 in medium (top panel) and lysates (second panel from the top) of COS-1 cells overexpressing PLXDC2 alone (lane 2), PLXDC2 with BACE1 (lanes 3 and 4), and PLXDC2 with BACE2 (lanes 5 and 6). Lanes 4 and 6 were treated with inhibitor CpJ (10 μM). Lane 1 was mock-transfected with an empty vector. Control blots for BACE1, BACE2, and actin are shown for each transfection. Representative for three experiments.
Iryna Voytyuk et al. LSA 2018;1:e
© 2018 Voytyuk et al.