1. Fig. 3 Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART selectively reverses baseline blocks to splicing, elongation, and completion.
Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART selectively reverses baseline blocks to splicing, elongation, and completion. The effects of activation were also measured using the ratio of one HIV transcript to another, which is independent of normalization to cell numbers. (A to D) Ratios to total (TAR) transcripts. HIV transcript measures in the unstimulated (day 0) and activated (days 1 and 2) CD4+ T cells were normalized to TAR to express the proportion of all HIV transcripts that are read-through (A), elongated (B), polyadenylated (C), and multiply spliced Tat-Rev (D) transcripts. (E to G) Ratios to long LTR. HIV transcript measures were normalized to long LTR to express the proportion of elongated HIV transcripts that are read-through (E), polyadenylated (F), and multiply spliced Tat-Rev (G) transcripts. (H) Ratio to polyadenylated. The ratio of Tat-Rev to polyA was used to express the proportion of completed transcripts that get multiply spliced to produce two-exon Tat-Rev. (I) Proportion of HIV transcripts blocked at the stages of transcriptional elongation, completion, and multiple splicing in unstimulated (day 0) and activated (day 2) CD4+ T cells. Each color represents a separate individual; comparisons were performed using the Wilcoxon signed-rank test.
Steven A. Yukl et al., Sci Transl Med 2018;10:eaap9927
Published by AAAS