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MET Increases the Sensitivity of Gefitinib-Resistant Cells to SN-38, an Active Metabolite of Irinotecan, by Up-Regulating the Topoisomerase I Activity 

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Presentation on theme: "MET Increases the Sensitivity of Gefitinib-Resistant Cells to SN-38, an Active Metabolite of Irinotecan, by Up-Regulating the Topoisomerase I Activity "— Presentation transcript:

1 MET Increases the Sensitivity of Gefitinib-Resistant Cells to SN-38, an Active Metabolite of Irinotecan, by Up-Regulating the Topoisomerase I Activity  Asao Sakai, MD, Kazuo Kasahara, PhD, Tohru Ohmori, PhD, Hideharu Kimura, PhD, Takashi Sone, PhD, Masaki Fujimura, PhD, Shinji Nakao, PhD  Journal of Thoracic Oncology  Volume 7, Issue 9, Pages (September 2012) DOI: /JTO.0b013e31825cca4c Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

2 Figure 1 The sensitivity of PC-9 and gefitinib-resistant PC-9/Met cells to gefitinib and SN-38. The cells (2 × 103 cells/well) were seeded on 96-well plates, and after preincubation for 24 hours were exposed to various concentrations of each drug. After incubation for 72 hours, the growth inhibition rate was analyzed by a WST-1 assay as described in the Materials and Methods section. The points represent the means of the data generated from at least three experiments performed in triplicate. Bars, SD. PC-9 cells, PC-9/Met cells. SN-38, 7-ethyl-10-hydroxy-camptothecin. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e31825cca4c) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

3 Figure 2 A, A comparison of the protein expression levels of topo I, MET, p-MET, EGFR, p-EGFR, Akt, and p-Akt in the PC-9 and PC-9/Met cells as determined by a Western blot analysis. Topo I migrated to approximately the 70 kDa position. The data are expressed as the means ± SD from more than three experiments. *p < B. The MET gene amplification status in the PC-9 and PC-9/Met cells was determined by a fluorescence in situ hybridization analysis. The chromosome 7 centromere signals are green, and the MET signals are red. MET amplification was found in the PC-9/Met cells, but not in the PC-9 cells. C, Topo I activity in the PC-9 cells, and the gefitinib-resistant PC-9/Met cells. Complete activity is seen when no supercoiled DNA substrate remains, and the presence of some supercoiled DNA indicates partial activity. The upper bands are attributable to DNA nicking by contaminating nucleases. The bottom band, indicated by the arrow, represents supercoiled DNA degraded by topo I. The topo I activity was normalized to the amount of nuclear protein extracted, such as 1.8 μg, 0.9 μg, 0.45 μg, and μg, respectively, in both cell lines. In the PC-9/Met cells, the band of supercoiled DNA was eliminated when 0.9 μg of nuclear protein was added to the reaction mixture. However, the band of supercoiled DNA remained when 1.8 μg of nuclear protein from the PC-9 cells was added. These results showed that the topo I activity of the PC-9/Met cells was approximately fourfold higher than that of the PC-9 cells. D, Measurement of the topo I mRNA expression in PC-9 and PC-9/Met cells as determined by real-time PCR. The topo I mRNA level of the PC-9/Met cells was approximately 9.2 times higher than that of the PC-9 cells. EGFR, epidermal growth receptor factor; p-EFGR, phospho-EGFR; p-Akt, phospho-Akt; p-MET, phospho-MET; topo I, topoisomerase I; mRNA, messenger RNA; PCR, polymerase chain reaction. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e31825cca4c) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

4 Figure 3 Specific down-regulation of MET affects the level of topo I protein expression and the enzyme activity, while also decreasing the sensitivity of cells to SN-38. Control and MET-specific siRNA were introduced into the PC-9 and PC-9/Met cell lines. A. Whole-cell proteins and nuclear proteins were extracted 48 hours later, and a Western blot analysis was performed. To detect the MET protein, a 20-μg protein sample was loaded per lane for the PC-9 cells and PC-9/Met cells. To measure topo I protein expression, 10-μg samples of nuclear protein were loaded for both the PC-9 and PC-9/Met cell lines. The data are expressed as the means ± SD from more than three experiments, *p < 0.05 versus control. B. The relationship between topo I expression and MET expression by a Western blotting analysis at 0 hour, 6 hours, 12 hours, and 24 hours after siRNA addition. C, The effect of the specific down-regulation of MET on the topo I activity of the PC-9/Met cells. MET-specific siRNA was introduced into PC-9/Met cells, and nuclear enzymes were extracted from the PC-9/Met cells that contained topo I. The PC-9/Met cells in which MET had been down-regulated had lower topo I activity than did the untreated PC-9/Met cells. D, The results of the WST-1 assay of cell-growth inhibition by SN-38. The PC-9/Met cells were more sensitive to SN-38 than the PC-9 cells, and the sensitivity of cells treated with MET siRNA was reduced. The points represent the means of data generated from at least three experiments performed in triplicate. Bars, SD. PC-9, PC-9/Met, PC-9/Met siRNA. topo I, topoisomerase I; siRNA, small interfering RNA, SN-38, 7-ethyl-10-hydroxy-camptothecin; con, control; scr, scramble. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e31825cca4c) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

5 Figure 4 A, Cell extracts from PC-9 cells and PC-9/Met cells were prepared after the cells were cultured with HGF (20 ng/ml) for 30 minutes and 60 minutes, after serum starvation for 24 hours. HGF stimulation induced MET phosphorylation in both cell lines. Nuclear extracts containing the topo I enzyme were prepared after 6 hours, 12 hours, and 24 hours of HGF stimulation, and the topo I protein expression level in both PC-9 and PC-9/Met cells was increased after HGF stimulation. The data are expressed as the means ± SD from three experiments, *p < 0.05 versus control. B, HGF increased the sensitivity of PC-9 and PC-9/Met cells to SN-38. Tumor cells at concentration of 2 × 104 cells/well were incubated in RPMI medium supplemented with 0.1% fetal bovine serum overnight. HGF (20 ng/ml) was added to the cultures of tumor cells for 4 hours. Increasing concentrations of SN-38 were added to the each well, and the incubation was continued for a further 24 hours. Then, the cell growth was determined by the water soluble tetrazolium salt-1 assay. HGF, hepatocyte growth factor; topo I, topoisomerase 1. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e31825cca4c) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

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