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Neointimal hyperplasia on a cell-seeded polytetrafluoroethylene graft is promoted by transfer of tissue plasminogen activator gene and inhibited by transfer.

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Presentation on theme: "Neointimal hyperplasia on a cell-seeded polytetrafluoroethylene graft is promoted by transfer of tissue plasminogen activator gene and inhibited by transfer."— Presentation transcript:

1 Neointimal hyperplasia on a cell-seeded polytetrafluoroethylene graft is promoted by transfer of tissue plasminogen activator gene and inhibited by transfer of nitric oxide synthase gene  Hong Yu, PhD, Wangde Dai, MD, PhD, Zhe Yang, MD, PhD, Rita L. Romaguera, MD, Paul Kirkman, Vincent L. Rowe, MD  Journal of Vascular Surgery  Volume 41, Issue 1, Pages (January 2005) DOI: /j.jvs Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

2 Fig. 1 Cell content in neointima. a, Density of seeded cells, which was calculated from lacZ positive cells in a measured area of neointima. b, Density of total cells, which was calculated from total cells counted in a measured area of neointima. P > .05 for all comparisons in (a) and (b) between different grafts (ANOVA). c, Total neointimal cells. Total cells in one section were calculated from the density of total cells multiplied by the area of neointima. The data are from 100-day explants. *P < .05 compared with type I graft. P > .05 for all other comparisons (ANOVA). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

3 Fig. 2 Immunostaining of neointima. Paraffin sections of grafts seeded with SMC/tPA (a), SMC/tPA+NOS (c), or SMC (b, d) were stained with antibody against human tPA (a, b) or antibody against human eNOS (c, d). Black arrows indicate positively stained cells in brown. White arrows indicate negtively stained cells with nuclear stained in blue. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

4 Fig. 3 Effect of tPA on thrombosis and neointima formation on PTFE graft. PTFE grafts (4 mm inside diameter) were seeded with no cell (I), SMC/lacZ (II), SMC/tPA (III), SMC/tPA+NOS (IV), or SMC/NOS (V) and then implanted into rabbit aorta as bypass grafts for 30 and 100 days. The recovered grafts were opened longitudinally (30 days, top panel). Thrombus (red) was observed on the luminal surface of type I and type II grafts. The grafts explanted at 100 days were patent except the graft seeded with SMC/tPA (middle panel). The sections of the grafts were stained with X-gal and hematoxylin-eosin to show the seeded cells (blue) and endogenous cells (pink) (bottom panels). Arrow 1, neointima; arrow 2, PTFE. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

5 Fig. 4 Cell migration through the PTFE wall. PTFE grafts seeded with SMC/lacZ were implanted into rabbit aorta for 30 days. After recovery, the grafts were sectioned and stained with x-gal and hematoxylin-eosin. a, Cells in adventitial surface (A) (red) infiltrated into luminal surface (L) of PTFE graft with porous structure (in brown). b, Seeded cells (blue) migrated from luminal surface to adventitial surface. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

6 Fig. 5 Analysis of neointima. Paraffin sections of grafts seeded with SMC (upper panel) or SMC/tPA (lower panel) were stained with hematoxylin-eosin (a, e), antibody for smooth muscle α–actin (b, f), Masson trichome (c, g), and antibody for PCNA (d, h). Nuclear are purple in hematoxylin-eosin staining; SMCs are brown in SMA staining; collagen is blue and cells are pink in trichome staining; nuclear of proliferating cells are dark blue in PCNA staining. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

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