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Priscilla Auyeung, PhD, Diana Mittag, PhD, Philip D

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Presentation on theme: "Priscilla Auyeung, PhD, Diana Mittag, PhD, Philip D"— Presentation transcript:

1 Autoreactive T cells in chronic spontaneous urticaria target the IgE Fc receptor Iα subunit 
Priscilla Auyeung, PhD, Diana Mittag, PhD, Philip D. Hodgkin, PhD, Leonard C. Harrison, DSc  Journal of Allergy and Clinical Immunology  Volume 138, Issue 3, Pages e4 (September 2016) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 T-cell proliferation to FcεRIα. A and B, CFSE-labeled PBMCs from a subject with CSU. Fig 1, A, Red rectangles: divided (CFSElo) CD4+ as percent of total CD4+ T cells. Fig 1, B, Antigen dose-response (mean ± SD of triplicates). C and D, CFSE-labeled PBMCs from CSU, DC, and HC subjects. Fig 1, C, Left: median CD4+ T-cell proliferation for individual subjects. Right: normalized data (background subtracted) to allow comparison between groups. Fig 1, D, CD8+ T-cell proliferation, as per subjects in Fig 1, C. Dotted lines: cutoffs for positive responses to FcεRIα (>2 interquartile ranges above the median HC value). Solid horizontal lines: group medians. CFSE, Carboxy-fluorescein diacetate succinimidyl ester. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 IFN-γ, IL-5, and IL-13 response to FcεRIα by ELISpot assay. A, PBMCs from subjects with CSU (mean ± SD of triplicates). B, PBMCs from CSU, DC, and HC subjects (median IFN-γ, IL-5, and IL-13 spots for individual subjects). C, Normalized data (background subtracted) to allow comparison between groups. Dotted lines: cutoffs for positive responses to FcεRIα (>2 interquartile ranges above the median HC value). Solid horizontal lines: group medians. aResponses to 0.1 vs 0 μM FcεRIα in each group were compared by using the Wilcoxon matched-pairs signed rank test. bBasal secretion between groups was compared by using the Kruskal-Wallis test. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Serum autoantibodies to FcεRIα. A, Immunoprecipitation of 125I-FcεRIα by autoantibodies in serum (1:100) from CSU, DC, and HC subjects. Dotted line indicates cutoff for a positive response to FcεRIα (>2 interquartile ranges above the median HC value). Solid horizontal lines denote group medians. B, Serum samples from 8 subjects with CSU were preincubated with unlabelled FcεRIα before incubation with 125I-FcεRIα. Median of triplicates shown for each subject. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 IFN-γ and autoantibody responses to FcεRIα. A, Comparison of IFN-γ and autoantibody responses to FcεRIα in 30 subjects with CSU. Median of replicates shown for each subject. B, Comparison of disease duration at the time of study in subjects with CSU with IFN-γ and autoantibody responses to FcεRIα. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 CD4+ T-cell proliferative, IFN-γ, IL-5 and IL-13 ELISpot, and autoantibody responses to FcεRIα in subjects with CSU. Data from 30 subjects with CSU. IFN-γ, IL-5, and IL-13 ELISpot data are combined. Percentages of subjects with responses are shown. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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