1. CD25 Blockade Delays Regulatory T Cell Reconstitution and Does Not Prevent Graft- versus-Host Disease After Allogeneic Hematopoietic Cell Transplantation 
Frederick L. Locke, Joseph Pidala, Barry Storer, Paul J. Martin, Michael A. Pulsipher, Thomas R. Chauncey, Niels Jacobsen, Nicolaus Kröger, Irwin Walker, Susan Light, Bronwen E. Shaw, Francisca Beato, Ginna G. Laport, Auayporn Nademanee, Armand Keating, Gerard Socie, Claudio Anasetti 
Biology of Blood and Marrow Transplantation 
Volume 23, Issue 3, Pages (March 2017)
DOI: /j.bbmt
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2. Figure 1
Cumulative incidence of grade III-IV acute GVHD (A) and chronic GVHD (B) according to treatment with placebo (arm A), daclizumab 0.3 mg/kg (arm B), or daclizumab 1.2 mg/kg (arm C).
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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3. Figure 2
Cumulative incidence curves for nonrelapse mortality (A) and relapse (B), and Kaplan-Meier curve for overall survival (C), according to treatment with placebo, daclizumab 0.3 mg/kg, or daclizumab 1.2 mg/kg.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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4. Figure 3
Flow cytometry analysis of lymphocyte subsets according to study arm. (A) Administration of daclizumab decreased the percentage of T cells expressing CD25 as measured by 7G7, a non–daclizumab-competing antibody against CD25, at days +11 to +35 and days +36 to +80, but not at days +81 to (B) Daclizumab administration decreased the percentage of T cells with free CD25-binding sites at days +11 to +35 and days +36 to +80. (C) Daclizumab was effectively bound to CD25+ T cells in vivo at days +11 to +35, days +36 to +90, and days +81 to (D) The percentage of Tregs in the CD4 T cell population (CD3+CD4+CD127lowCD25+FOXP3+) was decreased at days +11 to +35 and days +81 to (E) The percentage of CD4 cells with a central memory phenotype (CD3+CD4+CCR7+CD45RA-) was increased at 1 year (LTFU). (F) The percentage of CD8 cells with a central memory phenotype (CD3+CD8+CCR7+CD45RA-) was increased at days +81 to *P < .0001, 2-sample t-test. In all graphs, the box extends the interquartile range (IQR) from the 25th to 75th percentiles, the whiskers extend to the nearest data point 1.5 times the IQR beyond the 25th and 75th percentiles, and the dots represent data >1.5 times the IQR above the 75th quartile or below the 25th quartile. In some cases (C), the box and whiskers are all at 0 and not represented on the graph.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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