1. CD44 binding surface of the Tiam2 PHCCEx domain.
CD44 binding surface of the Tiam2 PHCCEx domain. (A) Mutated residues are mapped on the Tiam2 PHCCEx domain structure. Residues with detectable effects on CD44 binding are shown in red and residues with no effect on CD44 binding are shown in grey. (B) Sensor diagrams obtained from SPR measurements with the CD44 peptide (residues 327–350) immobilized on a sensor chip with injection of mutated Tiam2 PHCCEx domains (1 μM). (C) Mutation effects on KD values. Effects on CD44‐ and Par3‐binding affinity by mutations were estimated in terms of KD values determined by SPR measurements. (D) Model of membrane targeting by the PHCCEx domain. PI(4, 5)P2 is docked into the PH subdomain. Transmembrane proteins such as CD44 and ephrin B1 possess a unique acidic motif (orange) for binding to the PHCCEx domain.
Shin‐ichi Terawaki et al. EMBO J. 2010;29:
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