1. A minimal PALB2‐binding motif in the BRCA2 amino terminus.
A minimal PALB2‐binding motif in the BRCA2 amino terminus. (A) The previously localized PALB2‐binding site in residues 10–40 of BRCA2 (Xia et al, 2006) was mapped to residues 21–39, encapsulating a minimal ten‐residue motif, which is structured in the PALB2‐C–BRCA2(21–39) complex. (B) Biotinylated wild‐type BRCA2(21–39) peptide precipitates the PALB2‐C construct, confirming it as the BRCA2‐binding site. A peptide containing a breast‐cancer‐associated W31C mutation failed to co‐precipitate PALB2‐C. Bands marked with an asterisk arise from the NeutrAvidin resin. (C) Fluorescence polarization binding isotherm for interaction of PALB2‐C with fluorescein‐labelled BRCA2(21–39) peptide (see Methods). Kd as determined by nonlinear fitting of the data with a one‐site‐specific binding model (GraphPad Prism 5.0). BRCA2, breast cancer 2, early onset; PALB2, partner and localizer of BRCA2.
Antony W Oliver et al. EMBO Rep. 2009;10:
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