Presentation is loading. Please wait.

Presentation is loading. Please wait.

Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells  Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani,

Published byDiane Stephens Modified over 5 years ago

Similar presentations

Presentation on theme: "Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells  Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani,"— Presentation transcript:

1 Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells  Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani, MPH, DVM, Rajabrata Sarkar, MD, PhD, Theodore H. Welling, BS, Andrew Kriegel, MD, John W. Ford, BS, James C. Stanley, MD, Sem H. Phan, PhD, MD  Journal of Vascular Surgery  Volume 25, Issue 3, Pages (March 1997) DOI: /S (97) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Effect of TGF-β1 on lysyl oxidase enzyme activity secreted into media by cultured rat aortic smooth muscle cells. Confluent cultures were arrested in serum-free media for 24 hours and subsequently incubated for additional 24 hours in fresh, serum-free medium supplemented with 0.1, 0.5, 1.0, and 10 ng/ml of TGF-β1 or control vehicle. Data are pooled mean ± SEM of β-aminoproprionitrile-inhibited activity from triplicate aliquots of conditioned media derived from triplicate cultures for each condition. BAPN, β-aminoproprionitrile; TGF, transforming growth factor. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Concentration-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells incubated for 24 hours in presence of 0.1, 0.5, 1.0, and 10 ng/ml of TGF-β1 or control vehicle. Each lane represents RNA sample from single culture. B, Radioactive image analysis of Northern blot in A. Data are pooled mean ± SEM of activities from three lanes per condition. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 2 Concentration-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells incubated for 24 hours in presence of 0.1, 0.5, 1.0, and 10 ng/ml of TGF-β1 or control vehicle. Each lane represents RNA sample from single culture. B, Radioactive image analysis of Northern blot in A. Data are pooled mean ± SEM of activities from three lanes per condition. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 3 Time-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth-muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P-labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells cultured in presence of 1 ng/ml. B, Radioactive image analysis of Northern blot in A. Data are mean ± SEM for 2 or 3 lanes per condition. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 3 Time-dependent effect of TGF-β1 on steady-state levels of lysyl oxidase mRNA in cultured rat aortic smooth-muscle cells. Total cellular RNA was extracted, purified, and subjected to Northern blot analysis with 32P-labeled oligonucleotide probes complementary to unique sequence of cDNA for rat lysyl oxidase. A, Autoradiogram of Northern blot of total cellular RNA (15 μg/lane) derived from cells cultured in presence of 1 ng/ml. B, Radioactive image analysis of Northern blot in A. Data are mean ± SEM for 2 or 3 lanes per condition. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Download ppt "Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells  Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani,"

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.
The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.
Graft smooth muscle cells specifically synthesize increased collagen

Graft smooth muscle cells specifically synthesize increased collagen
Aortic injury occurring after minor trauma in ankylosing spondylitis

Aortic injury occurring after minor trauma in ankylosing spondylitis
Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion 

Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion 
Robert J. Pitsch, MD, David J. Minion, MD, Margaret L

Robert J. Pitsch, MD, David J. Minion, MD, Margaret L
Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,

Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,
Role of redox signaling and poly (adenosine diphosphate-ribose) polymerase activation in vascular smooth muscle cell growth inhibition by nitric oxide.

Role of redox signaling and poly (adenosine diphosphate-ribose) polymerase activation in vascular smooth muscle cell growth inhibition by nitric oxide.
Impact of a critical pathway on postoperative length of stay and outcomes after infrainguinal bypass  Andrew C. Stanley, MD, Maryann Barry, RN, Thayer.

Impact of a critical pathway on postoperative length of stay and outcomes after infrainguinal bypass  Andrew C. Stanley, MD, Maryann Barry, RN, Thayer.
Larry W. Kraiss, MDa,b,c, Neal M. Alto, BSa,c, Dan A

Larry W. Kraiss, MDa,b,c, Neal M. Alto, BSa,c, Dan A
Somatostatin gene transfer and expression in endothelial cells

Somatostatin gene transfer and expression in endothelial cells
A new valvulotome for in situ bypass grafts

A new valvulotome for in situ bypass grafts
Aortic injury occurring after minor trauma in ankylosing spondylitis

Aortic injury occurring after minor trauma in ankylosing spondylitis
Volume 114, Issue 3, Pages (March 1998)

Volume 114, Issue 3, Pages (March 1998)
Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD 

Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD 
In situ localization and quantification of seventy-two – kilodalton type IV collagenase in aneurysmal, occlusive, and normal aorta  William D. McMillan,

In situ localization and quantification of seventy-two – kilodalton type IV collagenase in aneurysmal, occlusive, and normal aorta  William D. McMillan,
Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone  C. Pross, MD,

Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone  C. Pross, MD,
Dennis R. Holmes, MD, William Wester, BA, Robert W

Dennis R. Holmes, MD, William Wester, BA, Robert W
Richard R. Keen, MD, Kevin D

Richard R. Keen, MD, Kevin D

Similar presentations

Ads by Google