1. Involvement of Protein Kinase A in Nitric Oxide Stimulating Effect on a BKCa Channel of Human Dermal Fibroblasts 
Siyoung Roh, Seyong Choi, Inja Lim 
Journal of Investigative Dermatology 
Volume 127, Issue 11, Pages (November 2007)
DOI: /sj.jid
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Effects of PKA inhibition on SNP-stimulated BKCa channel activity in cell-attached patches from human dermal fibroblasts. The membrane potential was -80mV. Bar at left indicates closed state. (a) Channel activity was recorded before (control) and after application of SNP. Subsequent addition of KT5720 (300nm) inhibited the SNP stimulatory effect on the NPo of BKCa channels (n=10, P<0.01). (b) Time-course plot of BKCa channel activity recorded from cell-attached patches. Channel activity during a 20-second pulse to -80mV is plotted as a bar on the graph. Lines above the graph indicate periods of drug exposure. Channel activity was before and after SNP (1mm) and KT5720 (300nm) with SNP. (c) SNP (1mm) made no significant increase in the NPo of BKCa channels after KT5720 (300nm) pretreatment for 20minutes (n=6).
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Effects of cAMP signaling pathway stimulation on BKCa channel activity in cell-attached patches from human dermal fibroblasts. The membrane potential was -80mV. Channel openings are upward deflections from baseline (bar on the left indicates closed state). (a) Forskolin (FSK) did not induce any significant increase in the NPo of BKCa channels (n=19). (b) 8-Br-cAMP also did not increase BKCa channel activity (n=9).
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
PKA stimulated BKCa channel activity in cell-free patches from human dermal fibroblasts. PKA catalytic subunit (PKAcs) alone did not increase the NPo of BKCa channel activity, but PKA together with cGMP produced a significant increase in the NPo of BKCa channel activity in (a) cell-attached patches (at -80mV, n=4) and (b) inside-out patches (at -30mV, n=5). Channel openings are upward deflections from baseline (bar on the left indicates closed state).
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Cross-talk effects between cAMP and cGMP signaling pathways on BKCa channel activity in cell-attached patches from human dermal fibroblasts. (a) Inhibition of sGC of the cGMP signaling pathway by ODQ inhibited the effects of PKAcs together with cGMP on the NPo of BKCa channels at -80mV (n=6, P<0.05). (b) Inhibition of PKA by KT5720 decreased the effect of 8-Br-cGMP on the BKCa channel activity (at -80mV, n=3). Channel openings are upward deflections from baseline (bar on the left indicates closed state).
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions