1. Signatures of activators and repressors
2-2 22
2-2 22
TF Expression
TF Expression
2-4 24
2-4 24
Motif enrichment
Motif enrichment
Motif enriched when
TF is expressed
Motif depleted when
TF is expressed
Active regions
Repressed regions

2. Signatures of +/- TF regulators: CREB / HNF
0: “Off” state
5,6: “Enhancer” states
9: “On” state
Repressor signature (CREB): When TF is expressed, motif is: Enriched in “off” states, Depleted in “on” states
0: “Off” state
5,6: “Enhancer” states
9: “On” state
Activator signature (HNF): When TF is expressed, motif is: Enriched in “on” states, Depleted in “off” states
2-2 22
Expression
2-4 24
Fold enrichment

3. Another activator: STAT
0: “Off” state
5,6: “Enhancer” states
9: “On” state
2-2 22
Expression
2-4 24
Fold enrichment
STAT: “Signal transducers and activator of transcription”
We see different enrichment and expression for both GM12878 and K562 lines with STAT1/5 in both enhancer and “on” states

4. Signatures of activators and repressors
Active states
2-2 22
TF Expression
+TF expressed  Motif depleted
2-4 24
Motif enrichment
Repressed states
TF expr  no motif
If motif  No expr
2-2 22
TF Expression
Activator signature +
2-4 24
Motif enrichment
+TF expressed  Motif enriched
2-2 22
TF Expression
Repressed states
-TF expressed  Motif enriched
2-4 24
Motif enrichment -
2-2 22
TF Expression
Active states
2-4 24
Motif enrichment
-TF expressed  Motif depleted
TF expr  no motif
If motif  No expr
Repressor signature

5. Example of activator and repressorxx
0: “Off” state
5,6: “Enhancer” states
9: “On” state
HNF HepG2 activator xx
0: “Off” state
5,6: “Enhancer” states
9: “On” state
CREB GM repressor
2-2 22
Expression
2-4 24
Fold enrichment