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VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells VAP silencing restores a normal plasma membrane cholesterol.

Published byChrista Elisabeth Mulder Modified over 5 years ago

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Presentation on theme: "VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells VAP silencing restores a normal plasma membrane cholesterol."— Presentation transcript:

1 VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells
VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells Plasma membrane cholesterol labeling using the GFP‐D4 probe as in Fig 8B. Live cells were incubated with GFP‐D4 probe (green) prior to fixation and nuclei staining (blue). Both signals are merged (a–i). Scale bar: 10 μm.Plasma membrane cholesterol was labeled using the GFP‐D4 probe. GFP‐D4 signal was quantified by flow cytometry. Mean ± SD; n = 4 independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001, ANOVA with Tukey's multiple comparison test.Indirect analysis of plasma membrane cholesterol content. Cells were treated with amphotericin B (20 μg/ml; 4 h at 37°C), and cell viability was measured using a luminescent cell viability assay. Relative cell viability is represented on the graph. Mean ± SD; n = 3; ***P < 0.001, ANOVA with Tukey multiple comparison test. Léa P Wilhelm et al. EMBO J. 2017;36: © as stated in the article, figure or figure legend

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