1. The peptide arrays stained with ...
The peptide arrays stained with pools of Th/To positive SSc serum samples (A, B) show several immunoreactive regions in all proteins. Reactivity with the pool of samples derived from SLE sera showed minor reactivity (C). Peptides that showed reactivity (>200 units) with at least one of the two Th/To serum pools, but not with the SLE pool are shown in D. In E, epitope map highlights main reactive peptides.
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2. The number of peptides that ...
The number of peptides that showed a reactivity of >200 units with at least one of the Th/To pools, but not with the SLE control pool, are displayed. For both Rpp25 and hPop1, more peptides reacted with pool 1, but not with pool 2. Rpp38 showed the highest number of peptides that reacted with both Th/To sample pools.
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3. The levels of anti-Rpp25 (recombinant ...
The levels of anti-Rpp25 (recombinant full-length protein) and anti-Rpp38 (the major Rpp38 peptide) antibodies are shown as measured by chemiluminescence immunoassay (CIA) in A. In B, reactivity is displayed according to the IIF pattern. The individual ICAP pattern as well as two groups [with (w) and without (w/o) any AC-08 pattern] are presented. lcSSc: limited cutaneous SSc; dcSSc: diffuse cutaneous); IIM: idiopathic inflammatory myopathies; HBV: hepatitis B virus; HCV: hepatitis C virus; HIV: human immunodeficiency virus; AC-01: homogeneous; AC-03: centromere; AC-04: fine speckled; AC-05: large coarse speckled; AC-08: homogeneous nucleolar; AC-09: clumpy nucleolar; AC-10: punctate nucleolar; AC-12: punctate nuclear envelop; AC-20: cytoplasmic fine speckled; AC-21: cytoplasmic anti-mitochondrial; AC-22: cytoplasmic Golgi-like.
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4. A: Correlation between anti-Rpp25 and anti-Rpp38 229–243 peptide ...
A: Correlation between anti-Rpp25 and anti-Rpp38 ^229–243 peptide antibodies. The reactivity between anti-Rpp25 and anti-Rpp38 peptide antibodies measured in 299 samples (203 SSc, 81 controls and 13 with unknown diagnosis) using a chemiluminescence assay showed significant correlation (rho = 0.44, 95% CI 0.35, 0.53; P < ). However, subsets of some patients either reacted with Rpp25 or with the novel Rpp38 derived peptide. B: Using a mixture of Rpp25 and Rpp38 coupled beads, samples that were positive for either of the two antigens were detected. All values on the ordinate axis are expressed as relative light units (RLU). QF: QUANTA Flash.
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