1. New methods for cooling and storing oocytes and embryos in a clean environment of −196°C 
Amir Arav, Yehudit Natan, Paolo Emanuele Levi-Setti, Francesca Menduni, Pasquale Patrizio 
Reproductive BioMedicine Online 
Volume 33, Issue 1, Pages (July 2016)
DOI: /j.rbmo
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2. Figure 1
CLAir®.
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3. Figure 2
Esther®, showing the canister and the specially designed top with the connected filter.
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4. Figure 3 The oxygen measuring system.
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5. Figure 4
Cooling rates measured in different volumes of cryogenic fluids (liquid air and liquid nitrogen). Cooling rates were measured three times at each volume. Different letters represent statistical differences; data is presented as mean ± SE (P < 0.05).
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6. Figure 5
(A) Temperature measurements of clean liquid air (50 ml) poured into a specially designed Styrofoam cup. Measurements were recorded every 10 s consecutively for 18 min and 50 s. (B) One-week temperature measurements recorded within the Esther® storage device.
Reproductive BioMedicine Online  , 71-78DOI: ( /j.rbmo )
Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions