1. High frequency and unique tumor‐initiating cells (TICs) in Pten/p53‐deficient mice Flow cytometry profiles of indicated tumors with the CD24 and CD49f cell surface markers.
High frequency and unique tumor‐initiating cells (TICs) in Pten/p53‐deficient mice Flow cytometry profiles of indicated tumors with the CD24 and CD49f cell surface markers. Gating conditions used to sort MMTV‐Cre:Ptenf/f:p53f/f tumor cells into CD24−:CD49f−, CD24−:CD49f+ and CD24+:CD49f+ fractions. CD24−:CD49f− cells contained significantly higher tumorsphere‐forming units (TFU) at a frequency of 0.23% (P <  , ANOVA with Tukey test for post hoc) compared with CD24−:CD49f+ or CD24+:CD49f+ fractions. The CD24−:CD49f+ fraction contained TFU at a frequency of 0.10% (P = 3.13 × 10−5, ANOVA with Tukey test for post hoc) relative to the CD24+:CD49f+ fraction. Representative images of tumorspheres from each fraction. TIC frequency in five MMTV‐Cre:Ptenf/f:p53f/f tumors following cell sorting and orthotopic transplantation into Rag1−/− females. There were too few cells in the CD24+:CD49f− fraction for analysis. Significant differences in TIC frequencies were determined using L‐Calc (Stemcell Technologies) and ANOVA. *P < 0.05; **P <  CD24/CD49f profiles of primary and secondary tumors from transplantation of CD24−:CD49f+ (blue) or CD24−:CD49f− (red) MMTV‐Cre:Ptenf/f:p53f/f tumor cells. Representative mesenchymal‐like histology of a primary MMTV‐Cre:Ptenf/f:p53f/f tumor and secondary tumors developed following transplantation of CD24−:CD49f+ or CD24−:CD49f− cell fractions.
Jeff C Liu et al. EMBO Mol Med. 2014;6:
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