1. A, Averaged full MS (ions converted to monoisotopic MW by Xcalibur Xtract) of Segment I-3 (see supplemental Fig.
A, Averaged full MS (ions converted to monoisotopic MW by Xcalibur Xtract) of Segment I-3 (seesupplementalFig. S3) of mAb digest. Peptides labeled with sequence identities are those picked for targeted MS/MS. The three groups of peptides colored green, red and blue correspond to three large peptides with potential N-glycans. B, ETD MS/MS of the peptide of 6708 Da MW in Fig. 4A. All of the ions in the original MS/MS spectrum were converted to +1 charge state by Xcalibur Xtract with c and z· fragments labeled. Below the spectrum is shown the sequence coverage and N-glycosylation assigned by ProSitePC 3.0 (with manual verification) based on the original MS/MS spectrum. More ions were labeled in the peptide sequence than in the spectrum because some fragment ions were lost in Xcalibur Xtract deconvolution.
Lichao Zhang et al. Mol Cell Proteomics 2016;15:
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.