1. Epitope Mapping Performance using a single peptide microarray.
Epitope Mapping Performance using a single peptide microarray.A, Protein Antigenicity profiles of three validated T. cruzi antigens: CA-2/B13, 60 S Ribosomal protein P2 and Ribosomal Protein L19. Horizontal axes show the amino acid positions of the protein sequences; vertical axes show the smoothed and normalized mean intensity values from a single peptide chip (sera pool C, replicate 1). In magenta: signal from negative sample (N). In black: cumulative signal from the negative and positive sample (NP). In green: Chagas-specific signal (subtraction of NP-N). Blue marks near the baseline show the position of known B-cell epitopes (antigenic regions within these antigens), as reported in the literature. B, Density plots showing the distribution of area under the ROC curve (AUC) values, where AUC = 1 means perfect residue by residue correspondence of signal with localization of previous known epitopes for 9 validation antigens. Color code for samples is the same as for panel A. Magenta: AUCs centered on 0.5, meaning no predictive performance. Black: average AUC = 0.861, for cumulative negative + positive signal. Green: AUC = 0.96, corresponding to Chagas-specific signal (for the single best performing assay).
Santiago J. Carmona et al. Mol Cell Proteomics 2015;14:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.