1. High-quality assembly of Dermatophagoides pteronyssinus genome and transcriptome reveals a wide range of novel allergens 
Xiao-Yu Liu, PhD, Kevin Yi Yang, PhD, Ming-Qiang Wang, MPhil, Jamie Sui-Lam Kwok, PhD, Xi Zeng, MSc, Zhiyuan Yang, MPhil, Xiao- Jun Xiao, PhD, Carol Po-Ying Lau, PhD, Ying Li, PhD, Zhi-ming Huang, BSc, Jin-ge Ba, BSc, Aldrin Kay-Yuen Yim, MPhil, Chun-Yan Ouyang, BSc, Sai-Ming Ngai, PhD, Ting-Fung Chan, PhD, Elaine Lai-Han Leung, PhD, Liang Liu, PhD, Zhi-Gang Liu, MD, Stephen Kwok-Wing Tsui, PhD 
Journal of Allergy and Clinical Immunology 
Volume 141, Issue 6, Pages e8 (June 2018)
DOI: /j.jaci
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2. Fig E1
Separation (left) and immunoblotting (right) of D pteronyssinus proteins from 2-dimensional-PAGE. Immunoblotting resulted in 50 protein spots in the total protein of the European HDM bound by specific IgE in the serum of the patients with HDM allergy, numbered 1 to 50. Note: The molecular weight of the separated proteins on the 2-dimensional gel ranged between 15 KDa and 100 KDa (left).
Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci )
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3. Fig E2
The allergenicity of the noncanonical allergens of Der p 25, 26, 28, 32, and 33 was detected by ELISA. ELISAs were performed with 26 sera of HDM allergy patients (1-26) and 4 normal human sera (N1-N4) as negative controls.
Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci )
Copyright © 2018 The Authors Terms and Conditions

4. Fig E3
Comparison of allergen expression levels between D pteronyssinus and D farinae. The gene expression levels were normalized by the β-actin gene. *Allergen numbers according to the systematic allergen nomenclature approved by the World Health Organization and International Union of Immunological Societies Allergen Nomenclature Subcommittee.
Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci )
Copyright © 2018 The Authors Terms and Conditions