1. Distributions of flagellar length in long-flagella mutants before and after F-L synchronization.
Distributions of flagellar length in long-flagella mutants before and after F-L synchronization. (a) lf3-2 mutant. (b) lf2-5 mutant. The upper parts of panels a and b represent the distributions of flagellar lengths in nonsynchronous cells. Cells were then deflagellated by acidic shock and regenerated for 4 h for F-L synchronization. The lower parts of panels a and b correspond to the distributions of flagellar lengths after F-L synchronization. n = 50/each (zeros excluded). The mean flagellar length of each mutant is marked by an asterisk. The green asterisks indicate the mean flagellar length observed in wild-type controls. (c) The mean flagellar lengths of wild-type and mutant cells were compared using Bonferroni’s post hoc test, and the results are represented. The first and second bars within each pair in panel c represent the mean flagellar lengths of nonsynchronized and synchronized cells, respectively. The Mann-Whitney U test was performed for comparing two means in a pair, and Bonferroni’s post hoc test was performed for multiple comparisons. Asterisks indicate significant differences (****, P ≤ ; **, P ≤ 0.01; ns, P > 0.05).
Soumita Dutta, and Prachee Avasthi mSphere 2017; doi: /mSphere