Presentation is loading. Please wait.

Presentation is loading. Please wait.

Indocyanine green angiography: A new method to quantify collateral flow in mice  Jan C. Wuestenfeld, MD, Joerg Herold, MD, Ulrike Niese, Utz Kappert, MD,

Published byHamdani Susman Modified over 5 years ago

Similar presentations

Presentation on theme: "Indocyanine green angiography: A new method to quantify collateral flow in mice  Jan C. Wuestenfeld, MD, Joerg Herold, MD, Ulrike Niese, Utz Kappert, MD,"— Presentation transcript:

1 Indocyanine green angiography: A new method to quantify collateral flow in mice 
Jan C. Wuestenfeld, MD, Joerg Herold, MD, Ulrike Niese, Utz Kappert, MD, Alexander Schmeisser, MD, Ruth H. Strasser, MD, Ruediger C. Braun-Dullaeus, MD  Journal of Vascular Surgery  Volume 48, Issue 5, Pages (November 2008) DOI: /j.jvs Copyright © Terms and Conditions

2 Fig 1 A, Hind limb perfusion in two regions of interest (red and green squares) of a (left) ligated and (right) unligated leg. A standard sponge (red arrow) and a reference area (white arrow) were simultaneously imaged to eliminate background signals. B, False-color images show the hind limb perfusion. High perfusion of the unligated leg and tail, and hypoperfusion of the ligated leg, were observed directly after occlusion. C, The influx velocity of indocyanine green, determined as the increase of fluorescence intensity in the regions of interest, is represented by the slope of the red (ligated area) and green (unligated area) curves. The standard sponge (yellow line) was used to eliminate background signals. The black bar indicates the time point of measurement for maximal fluorescence intensity after 20 seconds when maximal perfusion was observed in the unligated leg. D, Laser Doppler perfusion imaging of a mouse foot span demonstrates superficial skin perfusion 1 week after the ligation. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

3 Fig 2 Top, Time course of blood flow given as the perfusion index (slope of pixel intensity, ligated/unligated; blue bars) and maximal pixel intensity (ligated/unligated; red bars; n = 7). The green bars represent the results of laser Doppler perfusion imaging measurement. Error bars represent the standard error of the mean. A to E, Representative false-color images show the regions of interest in a ligated (red square) and unligated (green) hind limb. Mean signal intensity of the reference regions is green, lower fluorescence intensity is blue–green, and higher fluorescence intensities are gradually represented by yellow, orange, and red. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

4 Fig 3 Direct visualization of collateral arteries (white arrows) in representative (left) ligated and (right) unligated hind limbs during indocyanine green perfusion. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

5 Fig 4 Postmortem angiography of an (A) unligated and (B) ligated mouse hind limb. A few visible collaterals span the occlusion side (red arrows). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

6 Fig 5 Collateral arteries increase after ligation. The ligated/unligated ratio of collateral arteries was calculated for each animal (n = 7 for each group). Error bars designate the standard error of the mean. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

Download ppt "Indocyanine green angiography: A new method to quantify collateral flow in mice  Jan C. Wuestenfeld, MD, Joerg Herold, MD, Ulrike Niese, Utz Kappert, MD,"

Similar presentations

Assessment of mouse hind limb endothelial function by measuring femoral artery blood flow responses  Chao-Hung Wang, MD, Kuo-Ti Chen, MS, Hsiu-Fu Mei,

Assessment of mouse hind limb endothelial function by measuring femoral artery blood flow responses  Chao-Hung Wang, MD, Kuo-Ti Chen, MS, Hsiu-Fu Mei,
J. Dennis Baker, M. D. , Herbert I. Machleder, M. D

J. Dennis Baker, M. D. , Herbert I. Machleder, M. D
Education in vascular surgery: Critical issues in India

Education in vascular surgery: Critical issues in India
Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.
Bauer E. Sumpio, MD, PhD, Rachael O. Forsythe, MBChB, MRCS, Kenneth R

Bauer E. Sumpio, MD, PhD, Rachael O. Forsythe, MBChB, MRCS, Kenneth R
Successful lower extremity angioplasty improves brachial artery flow-mediated dilation in patients with peripheral arterial disease  Marc Husmann, MD,

Successful lower extremity angioplasty improves brachial artery flow-mediated dilation in patients with peripheral arterial disease  Marc Husmann, MD,
Assessment of peripheral skeletal muscle microperfusion in a porcine model of peripheral arterial stenosis by steady-state contrast-enhanced ultrasound.

Assessment of peripheral skeletal muscle microperfusion in a porcine model of peripheral arterial stenosis by steady-state contrast-enhanced ultrasound.
In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,
Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 
Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,
Hypertensive extracorporeal limb perfusion (HELP): A new technique for managing critical lower limb ischemia  Rodney J. Lane, MD, Mark Phillips, PhD,

Hypertensive extracorporeal limb perfusion (HELP): A new technique for managing critical lower limb ischemia  Rodney J. Lane, MD, Mark Phillips, PhD,
Gale Tang, MD, David N. Charo, Rong Wang, PhD, Israel F

Gale Tang, MD, David N. Charo, Rong Wang, PhD, Israel F
Coronary artery bypass grafting with the descending branch of thelateral femoral circumflex artery used as an arterial conduit: Is arteriographicevaluation.

Coronary artery bypass grafting with the descending branch of thelateral femoral circumflex artery used as an arterial conduit: Is arteriographicevaluation.
Extrinsic compression of the external iliac artery following internal fixation of an acetabular fracture  Erin Koelling, MD, Dipankar Mukherjee, MD  Journal.

Extrinsic compression of the external iliac artery following internal fixation of an acetabular fracture  Erin Koelling, MD, Dipankar Mukherjee, MD  Journal.
Robert A. Brenes, MD, Caroline C

Robert A. Brenes, MD, Caroline C
Combination of stromal-derived factor-1α and vascular endothelial growth factor gene- modified endothelial progenitor cells is more effective for ischemic.

Combination of stromal-derived factor-1α and vascular endothelial growth factor gene- modified endothelial progenitor cells is more effective for ischemic.
Peter F. Lawrence, MD, Olivia I

Peter F. Lawrence, MD, Olivia I
Ryan M. McEnaney, MD, Ankur Shukla, MD, Michael C

Ryan M. McEnaney, MD, Ankur Shukla, MD, Michael C
In vivo assessment of endothelial function in human lower extremity arteries  Vikram S. Kashyap, MD, Ryan O. Lakin, MD, Lindsay E. Feiten, BS, Paul D.

In vivo assessment of endothelial function in human lower extremity arteries  Vikram S. Kashyap, MD, Ryan O. Lakin, MD, Lindsay E. Feiten, BS, Paul D.
Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 

Similar presentations

Ads by Google