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Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin  Sonia Schuepbach-Mallepell, PhD, Virginie Philippe, PhD,

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Presentation on theme: "Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin  Sonia Schuepbach-Mallepell, PhD, Virginie Philippe, PhD,"— Presentation transcript:

1 Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin 
Sonia Schuepbach-Mallepell, PhD, Virginie Philippe, PhD, Marie-Charlotte Brüggen, MD, Hideki Watanabe, PhD, Stéphanie Roques, BSc, Christine Baldeschi, PhD, Olivier Gaide, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 132, Issue 6, Pages (December 2013) DOI: /j.jaci Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 The TH2 response is not affected by ASC or IL-1R deficiency in 2 AD models. A, Experimental scheme for Fig 1, B through E. B, Stronger response of BALB/c than C57Bl/6 mice to aDBP-FITC–induced AD. C, Response to aDBP-FITC in ASC- or IL-1R–deficient mice (n = 9, 3 independent experiments). D, aDBP-FITC–induced cytokine pattern (high IL-4/low IFN-γ = TH2 biased) in ASC- and IL-1R–deficient mice (n = 6, 2 independent experiments). E, T cell– and eosinophil-dominated skin infiltration after aDBP-FITC (n = 4). Bar = 0.2 mm. F, Ear swelling and IgE class-switching in a model of AD induced by DNCB (n = 9, 3 independent experiments). Error bars = SDs. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 DBP upregulates TSLP independently of the inflammasome, and both DNFB and IL-1β antagonize its induction. A, Experimental scheme for Fig 2, B through G. B, Real-time qPCR for mTSLP (n = 6, 2 independent experiments). C, TSLP levels in C57BL/6 versus BALB/c mice in response to aDBP. D, TSLP levels after aDBP stimulation in IL-1R– or ASC-deficient mice. E, Effect of IL-1β injection on TSLP mRNA levels (n = 6, 2 independent experiments). F, Effect of DNFB on basal and DBP-induced TSLP mRNA levels (n = 6, 2 independent experiments). G, Comparative TSLP mRNA levels in the epidermis and dermis of dispase-split ear skin (n = 3). H, TSLP mRNA levels in human skin xenotransplants (n = 2). Error bars = SDs. I, TSLP mRNA levels in human skin equivalents (epidermis only; n = 4). Error bars = SDs. J, TSLP protein expression in normal and AD human skin biopsy specimens at ×10 and ×40 magnification. Bars = 50 μm. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 IL-1 plays a key role in the negative regulation of TSLP by the inflammasome. TSLP mRNA levels 24 hours after 3 exposures to aDBP, DNFB, or both are shown. A, BALB/c mice: effect of the presence of IL-1R on TSLP levels. B, C57BL/6 mice: effect of the overexpression of transgenic IL-Ra on TSLP levels. C, BALB/C mice: effect of the intraperitoneal injection of recombinant IL-1Ra on TSLP levels. Error bars = SDs. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Negative regulation of TSLP expression by the inflammasome in cultured keratinocytes. TSLP mRNA levels after DBP, 9-cis-RA, tumor necrosis factor, IL-1β, or DNFB exposure. A, PAM212 murine keratinocytes. B, N-terts and HaCaT human keratinocytes. C, Primary murine keratinocytes. D, Primary human keratinocytes. E, MLE12 murine pneumocytes. F, A549 human pneumocytes. *P < .05 and **P < .01. Error bars = SDs. All experiments were done in triplicate in 3 or more independent experiments. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 DBP can redirect the DNTB response toward a TH2 response in mice. A, Ear swelling 24 or 48 hours after challenge of sensitized mice. B, RNA levels 24 hours after challenge showing the presence of T cells of the TH2 type (high IL-4/low IFN-γ) or TH1 type (opposite pattern). C, Skin peroxidase activity 24 hours after challenge as a measure of eosinophil infiltration (n = 6 in 2 independent experiments). Error bars = SDs. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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