1. Direct dFOXO targets in wild‐type adult flies.
Direct dFOXO targets in wild‐type adult flies. (A) Overlap between the genes that neighbour a dFOXO‐bound site and those with transcript levels altered in dfoxoΔ/Δ flies relative to wild type. For consistency with later experiments, both dfoxoΔ/Δ and wild‐type flies also carried the daGAL4 driver. The probability of overlap was calculated based on hypergeometric distribution and an overlap significantly larger than expected by chance (P<10−3) is indicated with a red asterisk. Note that only the dFOXO‐bound genes that were present on the expression arrays were taken into account. Representative biological functions enriched within the overlaps are shown. (B) dFOXO binding and regulation of IIS components. dFOXO binding and altered transcript levels in dfoxoΔ/Δ flies were mapped onto a schematic of IIS. Note that PI3K denotes the p110 subunit. (C) The levels of Serine 505‐phosphorylated AKT (pAKT) and the dually phosphorylated ERK (ppERK) were measured in wild‐type and dfoxoΔ/Δ females, as well as the levels of total AKT, ERK and dFOXO. dfoxoΔ/Δ females had 70% (±10%) of the wild‐type pAKT/AKT ratio, and 40% (±3%) of wild‐type ppERK/ERK. In both cases, the difference to wild type was significant (P<0.05, n=3, t‐test).
Nazif Alic et al. Mol Syst Biol 2011;7:502
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