1. Identification of progenitor states in human cord blood hematopoiesis
Identification of progenitor states in human cord blood hematopoiesis Schematic of experimental workflow, consisting of column‐based CD34 enrichment from human cord blood mononuclear cells, followed by Drop‐seq.Single‐cell heatmap of 19,394 cord blood progenitors. Shown are ten progenitor states identified from graph‐based clustering, and their strongest transcriptional markers. For visualization, expression for each gene is scaled (z‐scored) across single cells.Marker enrichment tests (Materials and Methods) comparing Drop‐seq clusters and reference cell types from Laurenti et al (2013). Our unbiased clustering recovered well‐characterized progenitor states, but we did not observe a cluster consistent with a traditional common myeloid progenitor (CMP).Compositional makeup of five independent cord blood units (CBUs). The width and color of each slice correspond to the percentage of cells in each CBU represented in each cluster.
Shiwei Zheng et al. Mol Syst Biol 2018;14:e8041
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