1. (A) Growth curves of strains at 30°C in SPP medium or SPP supplemented with 15 μM paclitaxel or 10 μM oryzalin.
(A) Growth curves of strains at 30°C in SPP medium or SPP supplemented with 15 μM paclitaxel or 10 μM oryzalin. Data represent mean values from three experiments and two experiments for oryzalin. For clarity, error bars are shown only for the last set of measurements. In panel A (left panel), the difference in the calculated generation time was significant with 95% confidence only between wild-type (WT) and double-knockout (KO) cells (P = 0.017). (B) Graph showing the rate of whole-cell motility expressed as the distance traveled by a cell within the time needed to collect five frames. Only the difference between wild-type and ATU1-6D cells was statistically significant (P < ). (C) Results of a phagocytosis assay. Averages from three experiments are shown, and 100 cells were scored for each time point. The percentage of cells with labeled (India ink) food vacuoles is indicated as a function of time after the addition of India ink. For the 2- and 5-min readouts, the differences between results obtained for wild-type and either double-knockout (P = ) or ATU1-6D (P = 0.005) cells were statistically significant. (D) Effects of gene knockouts on the average number of ciliary rows. Strains were analyzed at about 10 generations after their last mating. The results are mean values obtained for 50 cells. The differences between the results obtained with wild-type, TTLL1-null, and double-knockout or ATU1-6D cells were statistically significant (P = 0.001). All error bars represent standard deviations.
Dorota Wloga et al. Eukaryotic Cell 2008; doi: /EC