1. Detection of CSFV-specific antibodies in immunized/challenged pigs using blocking ELISA. Groups of pigs (n = 5) were inoculated with different doses of rPRVTJ-delgE/gI-E2, C-strain, or PBS and challenged with the CSFV Shimen strain at the indicated time points.
Detection of CSFV-specific antibodies in immunized/challenged pigs using blocking ELISA. Groups of pigs (n = 5) were inoculated with different doses of rPRVTJ-delgE/gI-E2, C-strain, or PBS and challenged with the CSFV Shimen strain at the indicated time points. Serum samples were collected at weekly intervals after immunization and at 2-day intervals after challenge (days 0 to 12) and tested for the presence of the CSFV-specific NAbs using an Idexx HerdChek CSFV antibody test kit (a blocking ELISA based on competition between peroxidase-labeled anti-E2 neutralizing MAb and serum NAbs for binding CFSV antigens), according to the manufacturer's instructions. Standard deviations are shown as error bars.
Yimin Wang et al. Clin. Vaccine Immunol. 2015; doi: /CVI