Presentation is loading. Please wait.

Presentation is loading. Please wait.

IL-18, but Not IL-12, Induces Production of IFN-γ in the Immunosuppressive Environment of HPV16 E7 Transgenic Hyperplastic Skin  Christina Gosmann, Ian.

Published byFanny Atmadjaja Modified over 5 years ago

Similar presentations

Presentation on theme: "IL-18, but Not IL-12, Induces Production of IFN-γ in the Immunosuppressive Environment of HPV16 E7 Transgenic Hyperplastic Skin  Christina Gosmann, Ian."— Presentation transcript:

1 IL-18, but Not IL-12, Induces Production of IFN-γ in the Immunosuppressive Environment of HPV16 E7 Transgenic Hyperplastic Skin  Christina Gosmann, Ian H. Frazer, Stephen R. Mattarollo, Antje Blumenthal  Journal of Investigative Dermatology  Volume 134, Issue 10, Pages (October 2014) DOI: /jid Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Elevated production of IFN-γ in K14E7 skin compared with wild-type skin. (a) IFN-γ concentrations in wild-type and K14E7 skin homogenates and supernatants of skin explants (four mice per group). Data points indicate individual mice analyzed in at least two independent experiments. (b, c) Flow cytometric analysis of cells isolated from dermis and epidermis of wild-type and K14E7 mice, unstimulated (unstim.) or stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin. (b) Total live cells gated for CD45.2+IFN-γ+ cells. Plots are representative of four independent experiments (two mice per strain per experiment). (c) Numbers of CD45.2+IFN-γ+ cells in wild-type and K14E7 dermis and epidermis obtained from four independent experiments. Bars indicate means±SEM. (d) IL-6 and IL-1β concentrations in wild-type and K14E7 skin homogenates (four mice per group). ISO, isotype control; NS, not significant; WT, wild type. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 The majority of IFN-γ-producing cells in K14E7 skin are CD8+ and CD4+ T cells. Flow cytometric analysis of cells isolated from the dermis and epidermis of K14E7 mice, stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin. (a, b) Percentage of CD3+, CD3+CD8+, and CD3+CD4+ T cells, iNKT cells, and γδ T cells of CD45+IFN-γ+ cells. Plots are representative of four independent experiments (two mice per experiment), for which results are summarized in b. (c) Mean fluorescence intensity of IFN-γ-producing cell subsets from four independent experiments. Bars indicate means±SEM of four independent experiments. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Elevated IL-12 production in K14E7 skin compared with wild-type skin is dispensable for IFN-γ production. (a) IL-12p70 and IL-12/23p40 concentrations in supernatants of C57BL/6 and K14E7 skin explants following 20 hours of culture (n=4 mice per group). Results are representative of at least two independent experiments. (b, c) IFN-γ concentrations in supernatants of K14E7 skin explants cultured with 10 μg ml-1 isotype, (b) anti-IL12/23p40 antibody, or (c) anti-IL-23p19 antibody (n=6 mice) for 40 hours. Results for individual mice obtained in two independent experiments are shown. (d) IFN-γ concentrations in the supernatants of K14E7 transgenic IL-12p40 heterozygous, IL-12p40-deficient, IFN-γ heterozygous (n=6–7 mice each), or IFN-γ-deficient (n=3 mice) skin explants (ND: below detection limit). Results were obtained in two independent experiments. Bars indicate means±SEM. ISO, isotype control; NS, not significant; WT, wild type. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 IL-18 production is elevated and induces IFN-γ production in K14E7 skin. (a) IL-18 mRNA expression in C57BL/6 and K14E7 skin (four mice per group). IL-18 concentrations in supernatants of C57BL/6 and K14E7 skin explants (20 hours; four mice per group). Means±SEM are indicated. (a, b) Western blots showing (a) pro-IL-18 (24 kDa), mature IL-18 (18 kDa) (n=3 mice), (b) pro-caspase-1 (45 kDa), and mature caspase-1 (10 kDa) (n=2 mice) in K14E7 skin lysates. β-Actin served as a loading control. Results are representative of at least two independent experiments. (c) IFN-γ concentrations in supernatants of K14E7 skin explants cultured with 10 μg ml-1 isotype or anti-IL-18 and/or anti-IL-12/23p40 antibodies for 40 hours. Data are paired observations for the same mouse, analyzed in two independent experiments (8–9 mice per treatment). ISO, isotype control; WT, wild type. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "IL-18, but Not IL-12, Induces Production of IFN-γ in the Immunosuppressive Environment of HPV16 E7 Transgenic Hyperplastic Skin  Christina Gosmann, Ian."

Similar presentations

In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.

In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.
CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens  Junpei Furuta, Takashi Inozume,

CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens  Junpei Furuta, Takashi Inozume,
Dermal Vγ4+ γδ T Cells Possess a Migratory Potency to the Draining Lymph Nodes and Modulate CD8+ T-Cell Activity through TNF-α Production  Satoshi Nakamizo,

Dermal Vγ4+ γδ T Cells Possess a Migratory Potency to the Draining Lymph Nodes and Modulate CD8+ T-Cell Activity through TNF-α Production  Satoshi Nakamizo,
Dissolving Microneedle Delivery of Nanoparticle-Encapsulated Antigen Elicits Efficient Cross-Priming and Th1 Immune Responses by Murine Langerhans Cells 

Dissolving Microneedle Delivery of Nanoparticle-Encapsulated Antigen Elicits Efficient Cross-Priming and Th1 Immune Responses by Murine Langerhans Cells 
Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes  Genji Imokawa, Yutaka Takagi,

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes  Genji Imokawa, Yutaka Takagi,
The Protective Effects of Melittin on Propionibacterium acnes–Induced Inflammatory Responses In Vitro and In Vivo  Woo-Ram Lee, Kyung-Hyun Kim, Hyun-Jin.

The Protective Effects of Melittin on Propionibacterium acnes–Induced Inflammatory Responses In Vitro and In Vivo  Woo-Ram Lee, Kyung-Hyun Kim, Hyun-Jin.
Volume 31, Issue 2, Pages (August 2009)

Volume 31, Issue 2, Pages (August 2009)
Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols Is Mediated Through DNA Repair and Subsequent Inhibition of Inflammation 

Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols Is Mediated Through DNA Repair and Subsequent Inhibition of Inflammation 
Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis  Gang Li, Adriana T. Larregina, Robyn.

Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis  Gang Li, Adriana T. Larregina, Robyn.
Oxidation of Cell Surface Thiol Groups by Contact Sensitizers Triggers the Maturation of Dendritic Cells  Saori Kagatani, Yoshinori Sasaki, Morihiko Hirota,

Oxidation of Cell Surface Thiol Groups by Contact Sensitizers Triggers the Maturation of Dendritic Cells  Saori Kagatani, Yoshinori Sasaki, Morihiko Hirota,
Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.
Trichomide A, a Natural Cyclodepsipeptide, Exerts Immunosuppressive Activity against Activated T Lymphocytes by Upregulating SHP2 Activation to Overcome.

Trichomide A, a Natural Cyclodepsipeptide, Exerts Immunosuppressive Activity against Activated T Lymphocytes by Upregulating SHP2 Activation to Overcome.
Down-Regulation of CD62L Shedding in T Cells by CD39+ Regulatory T Cells Leads to Defective Sensitization in Contact Hypersensitivity Reactions  Karsten.

Down-Regulation of CD62L Shedding in T Cells by CD39+ Regulatory T Cells Leads to Defective Sensitization in Contact Hypersensitivity Reactions  Karsten.
Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D  George W. Agak, Min Qin, Jennifer Nobe,

Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D  George W. Agak, Min Qin, Jennifer Nobe,
Generation of a Large Number of Connective Tissue Type Mast Cells by Culture of Murine Fetal Skin Cells  Nobuo Yamada, Hironori Matsushima, Yutaka Tagaya,

Generation of a Large Number of Connective Tissue Type Mast Cells by Culture of Murine Fetal Skin Cells  Nobuo Yamada, Hironori Matsushima, Yutaka Tagaya,
Absence of CCR4 Exacerbates Skin Inflammation in an Oxazolone-Induced Contact Hypersensitivity Model  Sari Lehtimäki, Sari Tillander, Anne Puustinen,

Absence of CCR4 Exacerbates Skin Inflammation in an Oxazolone-Induced Contact Hypersensitivity Model  Sari Lehtimäki, Sari Tillander, Anne Puustinen,
Vγ4 γδ T Cells Provide an Early Source of IL-17A and Accelerate Skin Graft Rejection  Yashu Li, Zhenggen Huang, Rongshuai Yan, Meixi Liu, Yang Bai, Guangping.

Vγ4 γδ T Cells Provide an Early Source of IL-17A and Accelerate Skin Graft Rejection  Yashu Li, Zhenggen Huang, Rongshuai Yan, Meixi Liu, Yang Bai, Guangping.
Langerhans Cells from Human Cutaneous Squamous Cell Carcinoma Induce Strong Type 1 Immunity  Hideki Fujita, Mayte Suárez-Fariñas, Hiroshi Mitsui, Juana.

Langerhans Cells from Human Cutaneous Squamous Cell Carcinoma Induce Strong Type 1 Immunity  Hideki Fujita, Mayte Suárez-Fariñas, Hiroshi Mitsui, Juana.
The Immune Response to Skin Trauma Is Dependent on the Etiology of Injury in a Mouse Model of Burn and Excision  Samantha M. Valvis, Jason Waithman, Fiona.

The Immune Response to Skin Trauma Is Dependent on the Etiology of Injury in a Mouse Model of Burn and Excision  Samantha M. Valvis, Jason Waithman, Fiona.
Circulating Th17, Th22, and Th1 Cells Are Increased in Psoriasis

Circulating Th17, Th22, and Th1 Cells Are Increased in Psoriasis

Similar presentations

Ads by Google