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The outline of each embryo sac is delimited using the freehand tool to create a region of interest (ROI) and saved using the ROI manager tool (Analyze>tools>ROI.

Published byEgbert Owens Modified over 5 years ago

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Presentation on theme: "The outline of each embryo sac is delimited using the freehand tool to create a region of interest (ROI) and saved using the ROI manager tool (Analyze>tools>ROI."— Presentation transcript:

1 The outline of each embryo sac is delimited using the freehand tool to create a region of interest (ROI) and saved using the ROI manager tool (Analyze>tools>ROI manager>Add).

2 In the “Analyze” menu, select “set measurements” and click on area and “mean gray value”.
Using the image Menu, select “color” and then “split channels”.

3 Close the image in the blue channel
Close the image in the blue channel. On the green channel, go to the analyze>tools>ROI manager and select the ROI added before. The ROI will appear on the image. Still in the ROI manager go to more>multi Measure. The result will appear in a new window.

4 Repeat this step with the image in the red channel.

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