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The usefulness of measuring tear periostin for the diagnosis and management of ocular allergic diseases  Hiroshi Fujishima, MD, Naoko Okada, PhD, Kenji.

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Presentation on theme: "The usefulness of measuring tear periostin for the diagnosis and management of ocular allergic diseases  Hiroshi Fujishima, MD, Naoko Okada, PhD, Kenji."— Presentation transcript:

1 The usefulness of measuring tear periostin for the diagnosis and management of ocular allergic diseases  Hiroshi Fujishima, MD, Naoko Okada, PhD, Kenji Matsumoto, MD, PhD, Kazumi Fukagawa, MD, PhD, Ayako Igarashi, BS, Akio Matsuda, PhD, Junya Ono, BS, Shoichiro Ohta, MD, PhD, Hideki Mukai, MD, PhD, Mamoru Yoshikawa, MD, PhD, Kenji Izuhara, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 138, Issue 2, Pages e2 (August 2016) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Elevation of IL-13 in tears of patients with AKC but not patients with VKC or SAC. A, Tear IL-13 in patients with AKC (n = 26), VKC (n = 4), and SAC (n = 15) and healthy donors (n = 14). B, Tear IL-13 in the large papilla formation group (scores 3-2, n = 20) and nonpapilla or less-papilla formation group (score 1-0, n = 6) in patients with AKC. C, Tear IL-13 in corneal damage-positive patients (n = 5) and corneal damage-negative patients (n = 17) in patients with AKC. ***P < .001 by Mann-Whitney U test. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Periostin production from conjunctival epithelial cells and fibroblasts by IL-13. POSTN mRNA (left panels) and periostin protein in supernatants (right panels) from conjunctival epithelial cells (upper panels) and fibroblasts (lower panels) stimulated without (white bars) or with (black bars) 10 ng/mL of IL-13 for the indicated times. Data are shown as the mean ± SD from fibroblasts from 5 separate donors and from epithelial cells from 3 separate donors. *P < .05 by Mann-Whitney U test. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Elevation of periostin in tears of patients with ocular allergic disease. A, Tear periostin in patients with AKC (n = 31), VKC (n = 7), and SAC (n = 17), healthy donors (n = 18), and patients with AR (n = 9) or AD (n = 16) without conjunctivitis. B, Correlation between periostin and IL-13 in tears of patients with ocular allergic disease and healthy controls (n = 59). C, Tear periostin in the large papilla formation group (scores 3-2, n = 23) and nonpapilla or less-papilla formation group (score 1-0, n = 8) in patients with AKC. D, Tear periostin in corneal damage-positive patients (n = 19) and corneal damage-negative patients (n = 8) in patients with AKC. E, Left: ROC curve between patients with AKC and patients with AD without conjunctivitis for periostin in tears. ROC curve between patients with allergic ocular disease versus healthy donors for periostin in tears. Right: ROC curve between corneal damage-negative (n = 8) and corneal damage-positive (n = 19) patients with AKC for periostin in tears. ROC curve between patients with chronic ocular allergic disease versus patients with SAC for periostin in tears. ROC, Receiver-operating characteristic. *P < .05, **P < .01, ***P < .001 by Mann-Whitney U test (A, C, D) or Spearman test (B). Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Expression of periostin in giant papilla formation. A, Staining of normal conjunctival tissues and giant papillae from patients with AKC with H&E and antiperiostin antibodies. The arrowheads indicate periostin staining. Scale bars: 50 μm. B and C, Expression of POSTN (Fig 4, B) or IL13 (Fig 4, C) at mRNA level relative to GAPDH mRNA in the giant papillae from patients with AKC (n = 15 or 14) or normal conjunctival tissues (n = 15 or 13). D, Correlation between POSTN and IL13 mRNA relative to GAPDH mRNA in the giant papillae from patients with AKC and normal conjunctival tissues (n = 27). ***P < .001 by Mann-Whitney U test (Fig 4, B and C) or Spearman test (Fig 4, D). H&E, Hematoxylin and eosin. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Correlation of serum and tear periostin in patients with ocular allergic disease. A, Serum periostin in patients with AKC (n = 22) and SAC (n = 12) and healthy donors (n = 13). B, Correlation of serum and tear periostin in patients with ocular allergic disease (n = 45). **P < .01, ***P < .001 by Mann-Whitney U test (Fig 5, A) or Spearman test (Fig 5, B). Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 Effects of topical treatment with tacrolimus on tear periostin in patients with AKC before and after topical treatment with 0.1% tacrolimus hydrate suspension (n = 8). Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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