1. Anti-inflammatory effects of DX-890, a human neutrophil elastase inhibitor 
Fiona K. Dunlevy, S. Lorraine Martin, Francine de Courcey, J. Stuart Elborn, Madeleine Ennis 
Journal of Cystic Fibrosis 
Volume 11, Issue 4, Pages (July 2012)
DOI: /j.jcf
Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

2. Fig. 1
NE activity from activated neutrophils. Effect of DX-890 (10nM, 100nM, 1μM) on NE activity from healthy (A) and CF (B) peripheral blood neutrophils activated with fMLP. Dotted line represents median spontaneous NE activity. Data are represented as median±interquartile range of n=5 (normal) and n=6 (CF) experiments. ⁎Wilcoxon signed rank p≤0.05.
Journal of Cystic Fibrosis  , DOI: ( /j.jcf )
Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

3. Fig. 2
Neutrophil transmigration. Effect of DX-890 (500nM) on transmigration of neutrophils through an epithelial monolayer in response to fMLP (100nM). Neutrophils that transmigrated to the lower chamber are expressed as a percentage of total neutrophil added to apical chamber. Triplicate values for n=4 experiments are presented as median and interquartile range. ⁎Wilcoxon signed rank test p≤0.05.
Journal of Cystic Fibrosis  , DOI: ( /j.jcf )
Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

4. Fig. 3
IL-8 release from epithelial cells in response to NE and CF sputum sol. IL-8 release from healthy and CF nasal epithelial cells in response to NE 50nM and CF sol 1:100. Data are median and interquartile range and are duplicate values for n=6 experiments. ⁎Wilcoxon signed rank test p≤0.005 compared to non-treated (normal cells). ΦWilcoxon signed rank test p≤0.005 compared to non-treated (CF cells).
Journal of Cystic Fibrosis  , DOI: ( /j.jcf )
Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

5. Fig. 4
Effect of DX-890 on IL-8 secretion induced by NE and CF sol. (A) DX-890 (200nM) reduces IL-8 release induced by NE 50nM from healthy and CF nasal epithelial cells. (B) DX-890 (500nM) reduces IL-8 release induced by CF sol 1:100 in healthy and CF nasal epithelial cells. Data are presented as median and interquartile range and are duplicate values for n=6 experiments. ⁎Wilcoxon signed rank test p≤0.05.
Journal of Cystic Fibrosis  , DOI: ( /j.jcf )
Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions