1. Volume 65, Issue 5, Pages 1959-1967 (May 2004)
Utility of endogenous creatinine clearance as a measure of renal function in mice 
Stephen R. Dunn, Zhonghua Qi, Erwin P. Bottinger, Matthew D. Breyer, Kumar Sharma 
Kidney International 
Volume 65, Issue 5, Pages (May 2004)
DOI: /j x
Copyright © 2004 International Society of Nephrology Terms and Conditions

2. Figure 1
Deproteinized mouse plasma, 3 μL injection. (A) Peak at 1.2 minutes is unknown. Creatinine appears as a singlet at 3.6 minutes. This peak represents a plasma creatinine value of 0.116mg/dL. (B) Deproteinized, diluted mouse urine. Peak at 3.6 minutes corresponds to creatinine. Earlier peaks are unknown.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

3. Figure 2
Comparison of creatinine levels. (A) Levels measured by Jaffé (Picric acid) method (•) and by high-performance liquid chromatography (HPLC) (○) in mouse plasma. Significantly higher creatinine levels in plasma (P < ) by Jaffé measurement are shown. (B) Same data plotted to show ratios. Jaffé overestimates creatinine in plasma, with values by Jaffé falling above the line of equivalence (dashed line). (C) Same data as a regression to demonstrate that a correlation between Jaffé and HPLC measurements (R = 0.738; P < 0.01) exists, but lack of sensitivity of Jaffé method is apparent.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

4. Figure 3
Comparison of creatinine levels. (A) Levels measured by Jaffé (Picric acid) method (▪) and by high-performance liquid chromatography (HPLC) (□) in mouse urine. A small but statistically significant difference is noted between HPLC and Jaffé creatinine measurements in urine (P = 0.01). The figure illustrates similarity between HPLC and Jaffé creatinine measurements. (B) Dashed line shows same data plotted with the line of equivalency. All values fall on or slightly above the line of equivalency consistent with a 10% overestimation by Jaffé. (C) Strong correlation between both methods for urine creatinine (R = 0.985, P < ). (D) Jaffé method underestimates creatinine clearance in mice. Ratios fall well below the line of equivalency.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

5. Figure 4
Plasma creatinine measurements. (A) High-performance liquid chromatography (HPLC) for C57BL/6J mice receiving a normal diet, high salt, and low salt diet + enalapril. (B) Corresponding creatinine clearance (HPLC). (C) The normalized creatinine clearances per gram of body weight.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

6. Figure 5
Plasma creatinine measurements. (A) High-performance liquid chromatography (HPLC) for C57BL/6J age/sex-matched normal mice (N = 8) and diabetic mice (N = 12). (B) Corresponding creatinine clearances (HPLC). (C) The normalized creatinine clearances per gram of body weight.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

7. Figure 6
Inulin clearance correlates with creatinine clearance when plasma is measured by high-performance liquid chromatography (HPLC).
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions