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SDS–PAGE and immunoblots of purified IgG for citrullination and carbamylation. a) Representative Coomassie-blue-stained isolated total IgG from patients and control sera used to blot for citrulline. b) Immunoblot of IgGs probed with anti-citrulline. c) Repr... SDS–PAGE and immunoblots of purified IgG for citrullination and carbamylation. a) Representative Coomassie-blue-stained isolated total IgG from patients and control sera used to blot for citrulline. b) Immunoblot of IgGs probed with anti-citrulline. c) Representative Coomassie-blue-stained isolated total IgG from patients and control sera used to blot for carbamylation. d) Immunoblot of IgGs probed with anti-carbamyl-lysine. Numerals correspond to patient identification codes in table 1. David Hutchinson et al. ERJ Open Res 2017;3: ©2017 by European Respiratory Society
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