1. Regulation of inflammatory and angiogenesis mediators in a functional model of decidualized endometrial stromal cells 
Amélie Bourdiec, Syed-Furquan Ahmad, Asmaa Lachhab, Ali Akoum 
Reproductive BioMedicine Online 
Volume 32, Issue 1, Pages (January 2016)
DOI: /j.rbmo
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2. Figure 1
Effect of decidualization on endometrial stromal cell receptivity and decidual marker expression. (A) Secretion of prolactin was measured by enzyme-linked immunosorbent assay in the culture supernatants of the decidualized and non-decidualized (NDC) endometrial stromal cells. (B, C and D), mRNA expression levels of LIFR, HOXA10, and HOXA11, respectively, were assessed by quantitative real-time polymerase chain reaction and normalized using glyceraldehyde-3-phosphate dehydrogenase. Student's t-test was used to compare the decidualized and control non-decidualized endometrial stromal cells (n = 5). Values are means ± SEM, *P < 0.05; **P < 0.01; ***P < DC = decidualized; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; LIFR = leukaemia inhibitory factor receptor; NDC = non-decidualized; PRL = prolactin.
Reproductive BioMedicine Online  , 85-95DOI: ( /j.rbmo )
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3. Figure 2
Effect of endometrial stromal cell decidualization on the expression of IL1 system members. mRNA expression levels of IL1R1, IL1R2 and IL1RN were assessed using quantitative real-time polymerase chain reaction and normalized using glyceraldehyde-3-phosphate dehydrogenase. IL1Rs, IL1RN and IL1B protein expression levels for the membrane-bound and intracytoplasmic forms in the total protein extracts and the soluble or secreted forms in the culture supernatants were determined by enzyme-linked immunosorbent assay. Student's t-test was used to the compare decidualized and control nondecidualized ESC cultures (n = 5). Values are means ± SEM, *P < 0.05; ***P < DC = decidualized; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; NDC = non-decidualized.
Reproductive BioMedicine Online  , 85-95DOI: ( /j.rbmo )
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

4. Figure 3
Effect of endometrial stromal cell decidualization on IL1 family members IL18 and IL33. mRNA expression levels of IL18R1 and ST2 were assessed by quantitative real-time polymerase chain reaction and normalized using glyceraldehyde-3-phosphate dehydrogenase. IL18Rs, ST2, IL18 and IL33 protein expression levels for the membrane-bound forms in total protein extracts and the soluble or secreted forms in the culture supernatants for were determined by enzyme-linked immunosorbent assay. Student's t-test was used to compare the decidualized and control non-decidualized endometrial stromal cell cultures (n = 5). Values are means ± SEM; *P < 0.05; **P < DC = decidualized; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; NDC = non-decidualized.
Reproductive BioMedicine Online  , 85-95DOI: ( /j.rbmo )
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

5. Figure 4
IL33 localization in proliferative and mid-secretory phase human endometrium. Representative photomicrographs showing immunohistochemical staining of IL33 in mid-secretory (A, B) and proliferative (C, D) phase human endometrium. Photomicrographs are representative of 5 tissues/group. Scale bars = 50 µm.
Reproductive BioMedicine Online  , 85-95DOI: ( /j.rbmo )
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6. Figure 5
Effect of endometrial stromal cell (ESC) decidualization on the secretion of angiogenic factors. Secretion of VEGF, TIMP3, MMP9, RANTES, MCP1 and MIF (A, B, C, D, E and F, respectively) was assessed in the culture supernatants of the decidualized or non-decidualized ESC using an enzyme-linked immunosorbent assay. Student's t-test was used to compare the decidualized and non-decidualized ESC (n = 5). Values are means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001; compared with non-decidualized ESC cultures using Student's t-test. DC = decidualized; MCP-1 = monocyte chemoattractant protein-1; MIF = migration inhibitory factor; MMP = matrix metallopeptidase; NDS = non-decidualized; RANTES = regulated on activation, normal T cell expressed and secreted; TIMP-3 = metallopeptidase inhibitor 3; VEGF = vascular endothelial cell growth factor.
Reproductive BioMedicine Online  , 85-95DOI: ( /j.rbmo )
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions