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Differential regulation of Akt phosphorylation in endometriosis

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1 Differential regulation of Akt phosphorylation in endometriosis
Ozgur Cinar, Yasemin Seval, Yesim H Uz, Hakan Cakmak, Murat Ulukus, Umit A Kayisli, Aydin Arici  Reproductive BioMedicine Online  Volume 19, Issue 6, Pages (December 2009) DOI: /j.rbmo Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

2 Figure 1 Immunolocalization of protein kinase B (pAkt) in human endometrium. Normal endometrium from early proliferative (A) and early secretory (B) phases. Eutopic endometrium from early proliferative (C) and early secretory (D and G) phases. Ectopic endometrium from early proliferative (E) and early secretory (F) phases. Immunofluorescent staining of pAkt in cultured endometriotic stromal cells after 2min oestradiol (10−8mol/l) exposure (H). Red signals indicate pAkt immunostaining and blue signals indicate nucleus with Hoechst Gl.Ep.=glandular epithelium, arrow=positive stained glandular epithelium, arrowhead (black)=positive stained stromal cell/s, arrowhead (white)=perinuclear pAkt immunopositivity. Bar=200μm for A–F, 40μm for G and 7μm for H. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

3 Figure 2 Histological scoring system (HSCORE) analysis of protein kinase B (pAkt) immunostaining in stromal and glandular cells. (A) Comparison of the HSCOREs in normal and eutopic endometrial tissues according to menstrual cycle phases. (B) Comparison of the HSCOREs in normal, eutopic and ectopic paired of eutopic endometrial stromal and glandular cells. Values are expressed as means±SEM. P<0.05 for (A) a, b, c, d, e, f versus a′, b′, c′, d′, e′, f′ and (B) a, b versus a′, b′ respectively. EGC=endometrial glandular cells; ESC=endometrial stromal cells. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

4 Figure 3 Western blot analysis of phosphorylated protein kinase B (pAkt) and total Akt (tAkt) in untreated normal and eutopic endometrial stromal cells in culture. Experiments were repeated three times with similar results and values are expressed as means±SEM. ∗P<0.05. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

5 Figure 4 Western blot analysis of oestradiol (10−8mol/l)-induced protein kinase B (Akt) phosphorylation in cultured normal and endometriotic stromal cells in a time-dependent (0–30min) manner. Experiments were repeated three times with similar results and values are expressed as means±SEM. ∗P<0.05. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

6 Figure 5 Analysis of the temporal effects (5–10min) of oestradiol (E2; 10−8mol/l), oestrogen receptor inhibitor ICI (10−6mol/l) on protein kinase B (Akt) phosphorylation in eutopic endometriosis cells (V=vehicle). ∗P<0.05, oestradiol and/or ICI treated cells versus control cells. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions


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