1. Investigation of the human stem cell factor KIT ligand gene, KITLG, in women with 46,XX spontaneous premature ovarian failure 
Emily S. Hui, B.A., Ekemini A. Udofa, Jackeline Soto, B.S., Vien H. Vanderhoof, C.R.N.P., Keith Zachman, M.S., Zhi-Bin Tong, M.D., Lawrence M. Nelson, M.D. 
Fertility and Sterility 
Volume 85, Issue 5, Pages (May 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Polymerase chain reaction amplification of the 10 exons of human KITLG.Human genomic DNA spanning each of the human KITLG exons was amplified by using intronic primers. Polymerase chain reaction products of the exons each were separated on 2% agarose gel electrophoresis and stained with ethidium bromide. The numbers above the PCR products represent the exon number. Size markers for DNA (φ X174 RF DNA/HaeIII) are shown at the left of the panel (M).
Hui. KITLG and premature ovarian failure. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Single-stranded conformation polymorphism analysis of KITLG exon 7 PCR products. The PCR products for exon 7 were separated by 20% TBE–acrylamide gel electrophoresis and stained with ethidium bromide. Single-stranded conformation polymorphism analysis of these samples from four patients with spontaneous premature ovarian failure and three normal control women are shown. The DNA mobility of PCR products from exon 7 was altered in one patient (arrows, lane 1). The DNA mobility of samples from normal women (lanes 5–7) and from patients with no changes in DNA mobility (lanes 2–4) also is seen.
Hui. KITLG and premature ovarian failure. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Analysis of DNA sequence of a KITLG coding region variant in exon 7. Electropherogram showing the sequence of the KITLG variant of exon 7 compared with the sequence of the wild type. The arrow indicates nucleotide 811 with the G→T polymorphism.
Hui. KITLG and premature ovarian failure. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Restriction fragment length polymorphism analysis of the KITLG variant in exon 7 using HinfI. Undigested wild-type DNA gives a single band of 360 base pairs. Upon digestion with HinfI enzyme, wild-type DNA yields two fragments, sized 265 and 95 base pairs. A heterozygote carrier will show all three fragment lengths of DNA upon HinfI digestion, as seen in the patient with a variant in exon 7.
Hui. KITLG and premature ovarian failure. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions