1. Effects of GM1 on astroglial and microglial markers
Effects of GM1 on astroglial and microglial markers R6/2 and WT mice were treated with artificial cerebro‐spinal fluid (CSF, vehicle) or GM1 for 28 days. ARepresentative brain section staining with anti‐GFAP antibodies. Areas shown are in the corpus striatum. Scale bars are 50 μm in length.BGraphs show the quantification of GFAP‐immunoreactive area in micrographs of coronal serial sections. For each mouse, eight serial sections were analysed and averaged. N = 11 WT CSF, 9 WT GM1, 10 R6/2 CSF, 8 R6/2 GM1.CGFAP protein expression in tissue lysates. Representative immunoblots and densitometric analysis are shown. N = 7 WT CSF, 7 WT GM1, 7 R6/2 CSF, 7 R6/2 GM1. The immunoblot showing α‐tubulin in the cortex is the same as for the cortex in (F), since GFAP and Iba1 were run in the same gel.D, ERepresentative micrographs (D) (from the striatum) and quantification (E) of Iba1+ cell density in the cortex and striatum. For each mouse, eight serial sections were analysed and averaged. Scale bars are 50 μm in length. N = 11 WT CSF, 10 WT GM1, 10 R6/2 CSF, 9 R6/2 GM1.FIba1 protein expression in tissue lysates. Representative immunoblots and densitometric analysis are shown. The immunoblot showing α‐tubulin in the cortex is the same as for the cortex in (C), since GFAP and Iba1 were run in the same gel. N = 7 WT CSF, 7 WT GM1, 6 R6/2 CSF, 7 R6/2 GM1.GSpi‐1 gene expression in the striatum, analysed by qPCR and normalized over the geometric mean of three stably expressed reference genes. N = 3 WT CSF, 3 WT GM1, 5 R6/2 CSF, 4 R6/2 GM1.Data information: Box‐and‐whisker plots show median, maximum and minimum values. Two‐way ANOVA with Holm–Sidak post‐test. *P < 0.05; **P < 0.01.Source data are available online for this figure.
Melanie Alpaugh et al. EMBO Mol Med. 2017;emmm
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