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Nucleic Acids Information storage 2006-2007.

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Presentation on theme: "Nucleic Acids Information storage 2006-2007."— Presentation transcript:

1 Nucleic Acids Information storage

2 Nucleotides 3 parts nitrogen base (C-N ring) pentose sugar (5C)
ribose in RNA deoxyribose in DNA phosphate (PO4) group Nitrogen base I’m the A,T,C,G or U part! Are nucleic acids charged molecules? DNA & RNA are negatively charged: Don’t cross membranes. Contain DNA within nucleus Need help transporting mRNA across nuclear envelope. Also use this property in gel electrophoresis.

3 Types of nucleotides 2 types of nucleotides different nitrogen bases
Purine = AG Pure silver! 2 types of nucleotides different nitrogen bases purines double ring N base adenine (A) guanine (G) pyrimidines single ring N base cytosine (C) thymine (T) uracil (U)

4 Building the polymer

5 Dangling bases? Why is this important?
Nucleic polymer Backbone sugar to PO4 bond phosphodiester bond new base added to sugar of previous base polymer grows in one direction N bases hang off the sugar-phosphate backbone Dangling bases? Why is this important?

6 Pairing of nucleotides
Nucleotides bond between DNA strands H bonds purine :: pyrimidine A :: T 2 H bonds G :: C 3 H bonds The 2 strands are complementary. One becomes the template of the other & each can be a template to recreate the whole molecule. Matching bases? Why is this important?

7 H bonds? Why is this important?
DNA molecule Double helix H bonds between bases join the 2 strands A :: T C :: G H bonds = biology’s weak bond • easy to unzip double helix for replication and then re-zip for storage • easy to unzip to “read” gene and then re-zip for storage H bonds? Why is this important?

8 Nucleic Acids Function: genetic material stores information
genes blueprint for building proteins DNA  RNA  proteins transfers information blueprint for new cells blueprint for next generation DNA proteins

9 Nucleic Acids Examples: Structure: RNA (ribonucleic acid)
single helix DNA (deoxyribonucleic acid) double helix Structure: monomers = nucleotides DNA RNA

10 Matching halves? Why is this a good system?
Copying DNA Replication 2 strands of DNA helix are complementary have one, can build other have one, can rebuild the whole when cells divide, they must duplicate DNA exactly for the new “daughter” cells Why is this a good system? Matching halves? Why is this a good system?

11 When does a cell copy DNA?
When in the life of a cell does DNA have to be copied? cell reproduction mitosis gamete production meiosis when cells divide, they must duplicate DNA exactly for the new “daughter” cells Why is this a good system?

12 DNA replication “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” James Watson Francis Crick 1953 The greatest understatement in biology!

13 Interesting note… Ratio of A-T::G-C affects stability of DNA molecule
2 H bonds vs. 3 H bonds biotech procedures more G-C = need higher T° to separate strands high T° organisms many G-C parasites many A-T (don’t know why) At the foundation of biology is chemistry!!

14 HELIXHELIX

15 Macromolecule Review

16 Proteins Structure / monomer Function Examples amino acids
levels of structure Function enzymes u defense transport u structure signals u receptors Examples digestive enzymes, membrane channels, insulin hormone, actin peptide bond

17 Lipids Structure / building block Function Examples
glycerol, fatty acid, cholesterol, H-C chains Function energy storage membranes hormones Examples fat, phospholipids, steroids ester bond (in a fat)

18 Carbohydrates Structure / monomer Function Examples monosaccharide
energy raw materials energy storage structural compounds Examples glucose, starch, cellulose, glycogen glycosidic bond

19 Nucleic acids Structure / monomer Function Examples nucleotide
information storage & transfer Examples DNA, RNA phosphodiester bond

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