1. Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization 
Yuri Mizusawa, M.D., Naoaki Kuji, M.D., Yudai Tanaka, M.D., Mamoru Tanaka, M.D., Eiji Ikeda, M.D., Setsuko Komatsu, Ph.D., Shingo Kato, Ph.D., Yasunori Yoshimura, M.D. 
Fertility and Sterility 
Volume 93, Issue 4, Pages (March 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Western blotting of extracts from unfertilized human oocytes (left lane) and human ovarian tissue (right lane). An identical band is seen at 36 kDa in each lane (arrow), corresponding to the molecular weight of hH1FOO protein.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Immunohistochemical analysis for hH1fOO in ovarian sections. Human ovarian follicles are shown at various stages, including a primordial follicle (a), primary follicle (b), secondary follicle (c), preantral follicle (d), and Graafian follicle (e, f). Staining is detected in both the nucleus (orange or dark yellow) and the cytoplasm (yellow) of the oocytes, with its intensity being greater in the nucleus.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
(A) Reverse transcription–nested PCR of hH1foo mRNA during oogenesis and early embryogenesis. hH1foo mRNA was detected at all stages from GV-stage oocytes to two-cell embryos as specific bands with a similar intensity. bp = base pair. (B) Fluorescence immunohistochemical analysis of an oocyte at the GV stage, an oocyte in MII, a PN oocyte, and a two-cell embryo (2-cell). The PN oocyte was obtained from IVF and ICSI, and the arrested 2PN oocyte was obtained after ICSI. The GV and MII oocytes were not exposed to sperm by either conventional IVF or ICSI. Deoxyribonucleic acid was visualized by Hoechst staining, and hH1FOO was detected by indirect immunofluorescence. hH1FOO protein can be detected clearly in the nucleus of the GV oocyte, MII oocyte, PN oocyte, and two-cell embryo. All polar bodies also were positive for both anti-hH1FOO antibody and Hoechst All of the sperm surrounding the PN oocyte, four-cell embryo, and morula were unstained by the anti-hH1FOO antibody (some are indicated by arrows). oc = oocyte chromatin; pb = polar body; pn = pronucleus; bc = blastomere chromatin.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Fluorescence immunohistochemical analysis of oocytes that were not fertilized by ICSI. These oocytes display three patterns of sperm chromatin immunoreactivity with the hH1foo antibody: [1] no immunoreactivity and no decondensation (A, B), [2] immunoreactivity without decondensation (C, D), and [3] immunoreactivity with decondensation (E, F). oc = oocyte chromatin; pb = polar body; sc = sperm chromatin.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions